Academic Journal
A yeast FRET biosensor enlightens cAMP signaling
| العنوان: | A yeast FRET biosensor enlightens cAMP signaling |
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| المؤلفون: | Botman, Dennis, O'Toole, Tom G., Goedhart, Joachim, Bruggeman, Frank J., van Heerden, Johan H., Teusink, Bas |
| المصدر: | Botman , D , O'Toole , T G , Goedhart , J , Bruggeman , F J , van Heerden , J H & Teusink , B 2021 , ' A yeast FRET biosensor enlightens cAMP signaling ' , Molecular Biology of the Cell , vol. 32 , no. 13 , pp. 1229-1240 . https://doi.org/10.1091/MBC.E20-05-0319 |
| سنة النشر: | 2021 |
| الوصف: | The cAMP-PKA signaling cascade in budding yeast regulates adaptation to changing environments. We developed yEPAC, a FRET-based biosensor for cAMP measurements in yeast. We used this sensor with flow cytometry for high-throughput single cell-level quantification during dynamic changes in response to sudden nutrient transitions. We found that the characteristic cAMP peak differentiates between different carbon source transitions and is rather homogenous among single cells, especially for transitions to glucose. The peaks are mediated by a combination of extracellular sensing and intracellular metabolism. Moreover, the cAMP peak follows the Weber-Fechner law; its height scales with the relative, and not the absolute, change in glucose. Last, our results suggest that the cAMP peak height conveys information about prospective growth rates. In conclusion, our yEPAC-sensor makes possible new avenues for understanding yeast physiology, signaling, and metabolic adaptation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1091/MBC.E20-05-0319 |
| الاتاحة: | https://research.vumc.nl/en/publications/e6a4fc30-8911-400e-a38f-cc5b84c85851 https://doi.org/10.1091/MBC.E20-05-0319 http://www.scopus.com/inward/record.url?scp=85108386575&partnerID=8YFLogxK |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.8AE8DCC7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1091/MBC.E20-05-0319 |
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