التفاصيل البيبلوغرافية
| العنوان: |
Proton and Water Transfer Pathways in the S 2 → S 3 Transition of the Water-Oxidizing Complex in Photosystem II: Time-Resolved Infrared Analysis of the Effects of D1-N298A Mutation and NO 3 – Substitution |
| المؤلفون: |
Yasutada Okamoto (11001171), Yuichiro Shimada (2220058), Ryo Nagao (1334679), Takumi Noguchi (1280298) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Medicine, Cell Biology, Genetics, Ecology, Sociology, Inorganic Chemistry, Infectious Diseases, Plant Biology, Computational Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, D 1-N mutation, N 298A PSII, proton transfer pathways, substitution, transition, water oxidation mechanism, Cl channels, Water Transfer Pathways, O 1 |
| الوصف: |
Photosynthetic water oxidation is performed through a light-driven cycle of five intermediates (S 0 –S 4 states) in photosystem II (PSII). The S 2 → S 3 transition, which involves concerted water and proton transfer, is a key process for understanding the water oxidation mechanism. Here, to identify the water and proton transfer pathways during the S 2 → S 3 transition, we examined the effects of D1-N298A mutation and NO 3 – substitution for Cl – , which perturbed the O1 and Cl channels, respectively, on the S 2 → S 3 kinetics using time-resolved infrared spectroscopy. The S 2 → S 3 transition was retarded both upon NO 3 – substitution and upon D1-N298A mutation, whereas it was unaffected by further NO 3 – substitution in N298A PSII. The H/D kinetic isotope effect in N298A PSII was relatively small, revealing that water transfer is a rate-limiting step in this mutant. From these results, it was suggested that during the S 2 → S 3 transition, water delivery and proton release occur through the O1 and Cl channels, respectively. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Proton_and_Water_Transfer_Pathways_in_the_S_sub_2_sub_S_sub_3_sub_Transition_of_the_Water-Oxidizing_Complex_in_Photosystem_II_Time-Resolved_Infrared_Analysis_of_the_Effects_of_D1-N298A_Mutation_and_NO_sub_3_sub_sup_sup_Substitution/14816238 |
| DOI: |
10.1021/acs.jpcb.1c03386.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.jpcb.1c03386.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.8A755DCB |
| قاعدة البيانات: |
BASE |