Academic Journal
Metabolic Responses of Lung Adenocarcinoma Cells to Survive under Stressful Conditions Associated with Tumor Microenvironment
| العنوان: | Metabolic Responses of Lung Adenocarcinoma Cells to Survive under Stressful Conditions Associated with Tumor Microenvironment |
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| المؤلفون: | Angeles Carlos-Reyes, Susana Romero-Garcia, Heriberto Prado-Garcia |
| المصدر: | Metabolites, Vol 14, Iss 2, p 103 (2024) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | tumor metabolism, tumor microenvironment, specific growth rate, lactic acidosis, hypoxia, glucose deprivation, Microbiology, QR1-502 |
| الوصف: | Solid tumors frequently present a heterogeneous tumor microenvironment. Because tumors have the potential to proliferate quickly, the consequence is a reduction in the nutrients, a reduction in the pH (<6.8), and a hypoxic environment. Although it is often assumed that tumor clones show a similar growth rate with little variations in nutrient consumption, the present study shows how growth-specific rate (µ), the specific rates of glucose, lactate, and glutamine consumption (qS), and the specific rates of lactate and glutamate production (qP) of 2D-cultured lung tumor cells are affected by changes in their environment. We determined in lung tumor cells (A427, A549, Calu-1, and SKMES-1) the above mentioned kinetic parameters during the exponential phase under different culture conditions, varying the predominant carbon source, pH, and oxygen tension. MCF-7 cells, a breast tumor cell line that can consume lactate, and non-transformed fibroblast cells (MRC-5) were included as controls. We also analyzed how cell-cycle progression and the amino acid transporter CD98 expression were affected. Our results show that: (1) In glucose presence, μ increased, but q S Glucose and q P Lactate decreased when tumor cells were cultured under acidosis as opposed to neutral conditions; (2) most lung cancer cell lines consumed lactate under normoxia or hypoxia; (3) although q S Glutamine diminished under hypoxia or acidosis, it slightly increased in lactate presence, a finding that was associated with CD98 upregulation; and (4) under acidosis, G0/G1 arrest was induced in A427 cancer cells, although this phenomenon was significantly increased when glucose was changed by lactate as the predominant carbon-source. Hence, our results provide an understanding of metabolic responses that tumor cells develop to survive under stressful conditions, providing clues for developing promising opportunities to improve traditional cancer therapies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2218-1989 |
| Relation: | https://www.mdpi.com/2218-1989/14/2/103; https://doaj.org/toc/2218-1989; https://doaj.org/article/d75875361dfe4de1acba2dfdb6a69898 |
| DOI: | 10.3390/metabo14020103 |
| الاتاحة: | https://doi.org/10.3390/metabo14020103 https://doaj.org/article/d75875361dfe4de1acba2dfdb6a69898 |
| رقم الانضمام: | edsbas.8A526A12 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 22181989 |
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| DOI: | 10.3390/metabo14020103 |