Academic Journal
Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica)
| العنوان: | Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica) |
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| المؤلفون: | Zheng, YT, Shaw, PC, Zhang, X, Sze, KH, Yang, Y, Ng, YM |
| بيانات النشر: | //www.elsevier.com/locate/yjsbi United States |
| سنة النشر: | 2011 |
| المجموعة: | University of Hong Kong: HKU Scholars Hub |
| مصطلحات موضوعية: | Luffin P1, Nuclear magnetic resonance, Anti-HIV-1, Ribosome-inactivating peptide, Luffa - chemistry, Anti-HIV Agents - chemistry - pharmacology, Peptides - chemistry - pharmacology, Seeds - chemistry, Circular Dichroism |
| الوصف: | Luffin P1, the smallest ribosome-inactivating peptide from the seeds of Luffa cylindrica was found to have anti-HIV-1 activity in HIV-1 infected C8166 T-cell lines and be able to bind with HIV Rev Response Element. Nuclear magnetic resonance spectroscopy revealed that the Luffin P1 comprises a helix-loop-helix motif, with the two alpha helices tightly associated by two disulfide bonds. Based on our findings, we conclude that unlike the well-studied ribosome-inactivating proteins, which exert their action through N-glycosidase activities, Luffin P1 demonstrates a novel inactivation mechanism probably through the charge complementation with viral or cellular proteins. Our work also provides a new scaffold for the design of novel inhibitors from a simple helical motif. © 2010 Elsevier Inc. ; link_to_subscribed_fulltext |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1047-8477 |
| Relation: | Journal of Structural Biology; Structural and functional studies of the SWIRM domain in Lysine-specific Demethylase 1 (LSD1); http://www.scopus.com/mlt/select.url?eid=2-s2.0-79952450056&selection=ref&src=s&origin=recordpage; Journal Of Structural Biology, 2011, v. 174 n. 1, p. 164-172; 8625089; 172; 185371; WOS:000288640100019; http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1047-8477&volume=174&issue=1&spage=164&epage=172&date=2011&atitle=Structural+characterization+and+anti-HIV-1+activities+of+arginine/glutamate-rich+polypeptide+Luffin+P1+from+the+seeds+of+sponge+gourd+(Luffa+cylindrica); eid_2-s2.0-79952450056; 164; http://hdl.handle.net/10722/133607; 174 |
| DOI: | 10.1016/j.jsb.2010.12.007 |
| الاتاحة: | https://doi.org/10.1016/j.jsb.2010.12.007 http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1047-8477&volume=174&issue=1&spage=164&epage=172&date=2011&atitle=Structural+characterization+and+anti-HIV-1+activities+of+arginine/glutamate-rich+polypeptide+Luffin+P1+from+the+seeds+of+sponge+gourd+(Luffa+cylindrica) http://hdl.handle.net/10722/133607 |
| Rights: | Appropriate Bibliographic Citation:Authors posting Accepted Author Manuscript online should later add a citation for the Published Journal Article indicating that the Article was subsequently published, and may mention the journal title provided that they add the following text at the beginning of the document: “NOTICE: this is the author’s version of a work that was accepted for publication in |
| رقم الانضمام: | edsbas.8A4222E1 |
| قاعدة البيانات: | BASE |
| تدمد: | 10478477 |
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| DOI: | 10.1016/j.jsb.2010.12.007 |