Academic Journal
Potential of Nanoparticles Chitosan for Delivery pcDNA3.1-SB3-HBcAg
| العنوان: | Potential of Nanoparticles Chitosan for Delivery pcDNA3.1-SB3-HBcAg |
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| المؤلفون: | Unsunnidhal Lalu, Jannah Raudatul, Haris Abdul, Supinganto Agus, Kusumawati Asmarani |
| المصدر: | BIO Web of Conferences, Vol 41, p 07003 (2021) |
| بيانات النشر: | EDP Sciences |
| سنة النشر: | 2021 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | a codon optimization, chitosan nanoparticles, hbcag, hepatitis b virus, delivery system, Microbiology, QR1-502, Physiology, QP1-981, Zoology, QL1-991 |
| الوصف: | Hepatitis B virus (HBV) is a DNA virus that causes hepatitis in humans. This study aims to prepare a Hepatitis DNA vaccine. he optimized base sequence of the SB3-HBcAg gene was derived from the nucleotide base sequence of the Hepatitis B core antigen B3 HBcAg subgenotype, and then Cloning of the pcDNA3.1-SB3-HBcAg has been successfully performed on E. coli DH5α and confirmed by PCR, restriction analysis and sequencing. The propagated plasmids were prepared as DNA-chitosan complex and physiochemical characterized using Particle Size Analyzer. Complex with a 4:1 (wt/wt) ratio of DNA with 0.04% concentration and chitosan have a mean diameter of 231.7 nm and zeta potential +12.3 mV and the value of Cytotoxicity Assay 80-90% as compared to the untreated cells that used as negative control, so it can be concluded that nanoparticles chitosan has good potential as a carrier agent for pcDNA3.1-SB3-HBcAg. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English French |
| تدمد: | 2117-4458 |
| Relation: | https://www.bio-conferences.org/articles/bioconf/pdf/2021/13/bioconf_biomic2021_07003.pdf; https://doaj.org/toc/2117-4458; https://doaj.org/article/7894b806dc494b01a0b1bca33e440818 |
| DOI: | 10.1051/bioconf/20214107003 |
| الاتاحة: | https://doi.org/10.1051/bioconf/20214107003 https://doaj.org/article/7894b806dc494b01a0b1bca33e440818 |
| رقم الانضمام: | edsbas.8A041621 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 21174458 |
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| DOI: | 10.1051/bioconf/20214107003 |