Academic Journal
Engineering of Bacillus megaterium for improving PHA production from glycerol
| العنوان: | Engineering of Bacillus megaterium for improving PHA production from glycerol |
|---|---|
| المؤلفون: | Gómez Cardozo, Javier Ricardo, Velasco-Bucheli, Rodrigo, Del Cerro, Carlos, De La Mata, I., Mora Martínez, Amanda Lucía |
| المساهمون: | Colciencias (Colombia), Ministerio de Ambiente y Desarrollo Sostenible (Colombia), Gómez Cardozo, Javier Ricardo, Velasco-Bucheli, Rodrigo, Del Cerro, Carlos, De La Mata, I., Mora Martínez, Amanda Lucía |
| بيانات النشر: | Malaysian Society of Molecular Biology & Biotechnology |
| سنة النشر: | 2019 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Bacillus megaterium, Glycerol, Glycerol dehydrogenase, Polyhydroxyalkanoates |
| الوصف: | 9 p.-4 fig. ; There are a few PHA-producer bacteria that can uptake glycerol to produce this biopolymer. Among them, Bacillus megaterium LVN01 has demonstrated to be able to grow up using glycerol as a carbon source. Glycerol dehydrogenase (GD) plays a key role in the synthesis of PHA from glycerol. In this study, the improvement of glycerol uptake by a recombinant strain of B. megaterium carrying pHT01-bmgd was evaluated in order to enhance PHA production. The biomass and PHA production were evaluated and compared to wild-type. It was determined that the PHA produced by both strains was PHB and the highest improvement in PHB yield was 226% at 30 h. ; This study was funded by Departamento Administrativo de Ciencia, Tecnología e Innovación de Colombia (COLCIENCIAS) (Called 647 of 2014). The authors would like to thank COLCIENCIAS for funding. This work is framed at the “Contrato de acceso a recursos genéticos y productos derivados del Ministerio del Medio Ambiente y Desarrollo Sostenible de Colombia Nº95 and Nº159”. ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0128-7451 2672-7277 |
| Relation: | Publisher's version; https://doi.org/10.35118/apjmbb.2019.027.3.07; Sí; AsPac J Mol Biol Biotechnol 27 (3) : 64-72 (2019); http://hdl.handle.net/10261/189298 |
| DOI: | 10.35118/apjmbb.2019.027.3.07 |
| الاتاحة: | http://hdl.handle.net/10261/189298 https://doi.org/10.35118/apjmbb.2019.027.3.07 |
| Rights: | open |
| رقم الانضمام: | edsbas.89C18C25 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 01287451 26727277 |
|---|---|
| DOI: | 10.35118/apjmbb.2019.027.3.07 |