Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis

التفاصيل البيبلوغرافية
العنوان:	Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis
المؤلفون:	Špak, J. (Josef), Votruba, I. (Ivan), Pavingerová, D. (Daniela), Holý, A. (Antonín), Špaková, V. (Vlastimila), Petrzik, K. (Karel)
سنة النشر:	2012
المجموعة:	The Czech Academy of Sciences: Publication Activity (ASEP) / Akademie věd ČR - Publikační činnost
مصطلحات موضوعية:	Caulimovirus, Chemotherapy, Pararetrovirus, dsDNA
الوصف:	Antiviral effects of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP, and ribavirin on double-stranded DNA Cauliflower mosaic virus (CaMV) were evaluated in Brassica pekinensis plants grown in vitro on liquid medium. A double-antibody sandwich ELISA was used for relative quantification of viral protein and PCR for detection of CaMV nucleic acid in plants. Ribavirin and PMEA had no significant antiviral effect. (S)-HPMPC at concentration 50 mg l -1 and PMEDAP at concentrations 50 and 12. 5 mg l -1 significantly (P < 0.05) reduced CaMV concentration in plants within 42-63 days to levels detectable neither by ELISA nor by PCR. A phytotoxicity experiment resulted in progressive yellowing of leaves and dwarfing in plants cultured 42 days on media with concentrations 12. 5, 25 and 50 mg l -1 of (S)-HPMPC and PMEDAP. Reduction in fresh and dry weights of plants was significant (P < 0. 05) already at 12. 5 mg l -1 with both compounds.
نوع الوثيقة:	article in journal/newspaper
اللغة:	English
تدمد:	0167-6857 1573-5044
Relation:	urn:pissn: 0167-6857; urn:eissn: 1573-5044; http://hdl.handle.net/11104/0209617
DOI:	10.1007/s11240-012-0130-0
الاتاحة:	https://doi.org/10.1007/s11240-012-0130-0 http://hdl.handle.net/11104/0209617
رقم الانضمام:	edsbas.88FDC8BA
قاعدة البيانات:	BASE

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الوصف
تدمد:	01676857 15735044
DOI:	10.1007/s11240-012-0130-0