Academic Journal
A Flow Cytometry-Based Serological Assay to Detect Visceral Leishmaniasis in HIV-Infected Patients
| العنوان: | A Flow Cytometry-Based Serological Assay to Detect Visceral Leishmaniasis in HIV-Infected Patients |
|---|---|
| المؤلفون: | da Silva, Elis D., de Oliveira, Beatriz C., Pereira, Allana M. de S., Guedes, Diego L., de Melo Neto, Osvaldo P., Costa, Carlos H. N., de Medeiros, Zulma M., Pereira, Valéria R. A. |
| المساهمون: | Conselho Nacional de Desenvolvimento CientÃfico e ógico, Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco |
| المصدر: | Frontiers in Medicine ; volume 8 ; ISSN 2296-858X |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2021 |
| المجموعة: | Frontiers (Publisher - via CrossRef) |
| الوصف: | Visceral Leishmaniasis (VL) is a severe parasitic disease that has emerged as an important opportunistic condition in HIV-infected patients and whose control is impaired by inaccurate identification. This is mainly due to the serological tests used for VL having a reduced performance in cases of VL-HIV coinfection due to a low humoral response. In this situation, however, a positive test has even greater diagnostic value when combined with the clinical status. This study aimed to evaluate the application and performance of flow cytometry to detect anti- Leishmania infantum antibodies in HIV-infected patients. Sera from VL/HIV coinfected patients, characterized using “gold standard” techniques, were compared with sera from healthy controls plus sera from HIV-infected individuals. The flow cytometry results were expressed as levels of IgG reactivity, based on the percentage of positive fluorescent parasites (PPFP). A ROC curve analysis of a serum titration indicated a PPFP of 1.26% as being the cutoff point to segregate positive and negative results. At the 1:2,048 dilution, with 89% sensitivity and 83% specificity, flow cytometry showed greater sensitivity in relation to the serological tests evaluated. Futhermore, flow cytometry was the only assay that positively identified all VL-HIV patients with quantified HIV load. Together, these findings suggest that flow cytometry may be used as an alternative serological approach for VL identification and as a tool to characterize the humoral response against Leishmania infantum in HIV-infected patients. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.3389/fmed.2021.553280 |
| DOI: | 10.3389/fmed.2021.553280/full |
| الاتاحة: | http://dx.doi.org/10.3389/fmed.2021.553280 https://www.frontiersin.org/articles/10.3389/fmed.2021.553280/full |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.88CDE86E |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fmed.2021.553280 |
|---|