التفاصيل البيبلوغرافية
| العنوان: |
Direct N- or C‑Terminal Protein Labeling Via a Sortase-Mediated Swapping Approach |
| المؤلفون: |
Min Cong (4354978), Soheil Tavakolpour (9280121), Lea Berland (11664102), Hannah Glöckner (11664105), Bohdan Andreiuk (11664108), Taha Rakhshandehroo (11664111), Safak Uslu (11664114), Shruti Mishra (9293587), Louise Clark (11664117), Mohammad Rashidian (1360473) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Microbiology, Genetics, Molecular Biology, Physiology, Biotechnology, Inorganic Chemistry, Infectious Diseases, Plant Biology, Virology, Computational Biology, Physical Sciences not elsewhere classified, prior modification beyond, specific protein modifications, specific protein modification, mediated swapping approach, specific protein labeling, sortase recognition motif, method allows rapid, terminal protein labeling, terminal labeling, protein engineering, straightforward approach, specific n, simultaneous labeling, quantitative yield, novel sortase, genetic incorporation, fusion proteins |
| الوصف: |
Site-specific protein labeling is important in biomedical research and biotechnology. While many methods allow site-specific protein modification, a straightforward approach for efficient N-terminal protein labeling is not available. We introduce a novel sortase-mediated swapping approach for a one-step site-specific N-terminal labeling with a near-quantitative yield. We show that this method allows rapid and efficient cleavage and simultaneous labeling of the N or C termini of fusion proteins. The method does not require any prior modification beyond the genetic incorporation of the sortase recognition motif. This new approach provides flexibility for protein engineering and site-specific protein modifications. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Direct_N-_or_C_Terminal_Protein_Labeling_Via_a_Sortase-Mediated_Swapping_Approach/16958844 |
| DOI: |
10.1021/acs.bioconjchem.1c00442.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.bioconjchem.1c00442.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.8889118E |
| قاعدة البيانات: |
BASE |