التفاصيل البيبلوغرافية
| العنوان: |
Data_Sheet_1_Proteome Modulation in Peripheral Blood Mononuclear Cells of Peste des Petits Ruminants Vaccinated Goats and Sheep.doc |
| المؤلفون: |
Sajad Ahmad Wani (4245814), Amit Ranjan Sahu (5999222), Raja Ishaq Nabi Khan (5999225), Manas Ranjan Praharaj (11465290), Shikha Saxena (5999231), Kaushal Kishor Rajak (5999240), Dhanavelu Muthuchelvan (11465293), Aditya Sahoo (11465296), Bina Mishra (5999246), R. K. Singh (2147218), Bishnu Prasad Mishra (5999249), Ravi Kumar Gandham (4245802) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Animal Systematics and Taxonomy, Animal Physiology - Biophysics, Animal Physiology - Cell, Animal Physiology - Systems, Animal Behaviour, Animal Cell and Molecular Biology, Animal Developmental and Reproductive Biology, Animal Immunology, Animal Neurobiology, Animal Physiological Ecology, Animal Structure and Function, Veterinary Anaesthesiology and Intensive Care, Veterinary Anatomy and Physiology, Veterinary Diagnosis and Diagnostics, Veterinary Epidemiology, Veterinary Immunology, Veterinary Medicine, Veterinary Microbiology (excl. Virology), Veterinary Parasitology, Veterinary Pathology, Veterinary Pharmacology, Veterinary Surgery, Veterinary Virology, Veterinary Sciences not elsewhere classified, Sungri/96, PPRV, proteome, immunity, vaccine |
| الوصف: |
In the present study, healthy goats and sheep (n = 5) that were confirmed negative for peste des petits ruminants virus (PPRV) antibodies by monoclonal antibody-based competitive ELISA and by serum neutralization test and for PPRV antigen by s-ELISA were vaccinated with Sungri/96. A quantitative study was carried out to compare the proteome of peripheral blood mononuclear cells (PBMCs) of vaccinated goat and sheep [5 days post-vaccination (dpv) and 14 dpv] vs. unvaccinated (0 day) to divulge the alteration in protein expression following vaccination. A total of 232 and 915 proteins were differentially expressed at 5 and 14 dpv, respectively, in goats. Similarly, 167 and 207 proteins were differentially expressed at 5 and 14 dpv, respectively, in sheep. Network generated by Ingenuity Pathway Analysis was “infectious diseases, antimicrobial response, and inflammatory response,” which includes the highest number of focus molecules. The bio functions, cell-mediated immune response, and humoral immune response were highly enriched in goats at 5 dpv and at 14 dpv. At the molecular level, the immune response produced by the PPRV vaccine virus in goats is effectively coordinated and stronger than that in sheep, though the vaccine provides protection from virulent virus challenge in both. The altered expression of certain PBMC proteins especially ISG15 and IRF7 induces marked changes in cellular signaling pathways to coordinate host immune responses. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/Data_Sheet_1_Proteome_Modulation_in_Peripheral_Blood_Mononuclear_Cells_of_Peste_des_Petits_Ruminants_Vaccinated_Goats_and_Sheep_doc/16656709 |
| DOI: |
10.3389/fvets.2021.670968.s001 |
| الاتاحة: |
https://doi.org/10.3389/fvets.2021.670968.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.88812AEC |
| قاعدة البيانات: |
BASE |