Academic Journal
Fatty Acids Increase Neuronal Hypertrophy of PTEN Knockdown Neurons
| العنوان: | Fatty Acids Increase Neuronal Hypertrophy of PTEN Knockdown Neurons |
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| المؤلفون: | Catherine J Fricano, Tyrone eDeSpenza, Paul eFrazel, Meijie eLi, James eO'Malley, Gary L Westbrook, Bryan Weston Luikart |
| المصدر: | Frontiers in Molecular Neuroscience, Vol 7 (2014) |
| بيانات النشر: | Frontiers Media S.A. |
| سنة النشر: | 2014 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Dendrites, Dendritic Spines, Dentate Gyrus, Fatty Acids, Hypertrophy, autism, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571 |
| الوصف: | Phosphatase and tensin homolog (Pten) catalyzes the reverse reaction of PI3K by dephosphorylating PIP3 to PIP2. This negatively regulates downstream Akt/mTOR/S6 signaling resulting in decreased cellular growth and proliferation. Co-injection of a lentivirus knocking Pten down with a control lentivirus allows us to compare the effects of Pten knockdown between individual neurons within the same animal. We find that knockdown of Pten results in neuronal hypertrophy by 21 days post-injection. This neuronal hypertrophy is correlated to increased p-S6 and p-mTOR in individual neurons. We used this system to test whether an environmental factor that has been implicated in cellular hypertrophy could influence the severity of the Pten knockdown-induced hypertrophy. Implantation of mini-osmotic pumps delivering fatty acids results in increased neuronal hypertrophy and p-S6/p-mTOR staining. These hypertrophic effects were reversed in response to rapamycin treatment. However, we did not observe a similar increase in hypertrophy in response to dietary manipulations of fatty acids. Thus, we conclude that by driving growth signaling with fatty acids and knocking down a critical regulator of growth, Pten, we are able to observe an additive morphological phenotype of increased soma size mediated by the mTOR pathway. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1662-5099 |
| Relation: | http://journal.frontiersin.org/Journal/10.3389/fnmol.2014.00030/full; https://doaj.org/toc/1662-5099; https://doaj.org/article/7eb076927d3d4321a1dc6c9f1ba1edec |
| DOI: | 10.3389/fnmol.2014.00030 |
| الاتاحة: | https://doi.org/10.3389/fnmol.2014.00030 https://doaj.org/article/7eb076927d3d4321a1dc6c9f1ba1edec |
| رقم الانضمام: | edsbas.886E29CD |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 16625099 |
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| DOI: | 10.3389/fnmol.2014.00030 |