Academic Journal
Higher-order G-quadruplex structures and porphyrin ligands: Towards a non-ambiguous relationship
| العنوان: | Higher-order G-quadruplex structures and porphyrin ligands: Towards a non-ambiguous relationship |
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| المؤلفون: | Falanga, Andrea Patrizia, D'Urso, Alessandro, Travagliante, Gabriele, Gangemi, Chiara Maria Antonietta, Marzano, Maria, D'Errico, Stefano, Terracciano, Monica, Greco, Francesca, De Stefano, Luca, Dardano, Principia, Rea, Ilaria, Piccialli, Gennaro, Oliviero, Giorgia, Borbone, Nicola |
| المساهمون: | Falanga, Andrea Patrizia, D'Urso, Alessandro, Travagliante, Gabriele, Gangemi, Chiara Maria Antonietta, Marzano, Maria, D'Errico, Stefano, Terracciano, Monica, Greco, Francesca, De Stefano, Luca, Dardano, Principia, Rea, Ilaria, Piccialli, Gennaro, Oliviero, Giorgia, Borbone, Nicola |
| سنة النشر: | 2024 |
| المجموعة: | IRIS Università degli Studi di Napoli Federico II |
| مصطلحات موضوعية: | DNA-porphyrin complexe, G-quadruplex, G-wire, Polarity inversion, Porphyrin ligands |
| الوصف: | Herein, we evaluated the interaction of the tetracationic porphyrin H2TCPPSpm4 with three distinct DNA G-quadruplex (G4) models, i.e., the tetramolecular G4 d(TGGGGT)4 (Q1), the 5'-5' stacked G4-dimer [d(CGGAGGT)4]2 (Q2), and a mixture of 5'-5' stacked G-wires [d(5'-CGGT-3'-3'-GGC-5')4]n (Qn). The combined data obtained from UV-Vis, CD, fluorescence, PAGE, RLS, AFM, NMR, and HPLC-SEC experiments allowed us to shed light on the binding mode of H2TCPPSpm4 with the three G4 models differing for the type and the number of available G4 ending faces, the length of the G4 units, and the number of stacked G4 building blocks. Specifically, we found that H2TCPPSpm4 interacted with the shortest Q1 as an end-stacking ligand, whereas the groove binding mode was ascertained in the case of the Q2 and Qn G4 models. In the case of the interaction with Q1 and Qn, we found that H2TCPPSpm4 induces the formation of supramolecular aggregates at porphyrin/G4 ratios higher than 2:1, whereas no significant aggregation was observed for the interaction with Q2 up to the 5:1 ratio. These results unambiguously demonstrated the suitability of porphyrins for the development of specific G4 ligands or G4-targeting diagnostic probes, being H2TCPPSpm4 capable to distinguish between different G4s. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-14-181302-8 0-14-181302-4 |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:001237734700001; volume:268; issue:Pt 2; numberofpages:14; journal:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES; https://hdl.handle.net/11588/962946; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85192002252; https://www.sciencedirect.com/science/article/pii/S0141813024026060?via=ihub |
| DOI: | 10.1016/j.ijbiomac.2024.131801 |
| الاتاحة: | https://hdl.handle.net/11588/962946 https://doi.org/10.1016/j.ijbiomac.2024.131801 https://www.sciencedirect.com/science/article/pii/S0141813024026060?via=ihub |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.881059CD |
| قاعدة البيانات: | BASE |
| ردمك: | 9780141813028 0141813024 |
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| DOI: | 10.1016/j.ijbiomac.2024.131801 |