التفاصيل البيبلوغرافية
| العنوان: |
Epi-cDC2s are more extensively infected with HSV-1 than LCs. |
| المؤلفون: |
Kirstie M. Bertram (10711553), Naomi R. Truong (10711556), Jacinta B. Smith (10711559), Min Kim (218786), Kerrie J. Sandgren (10711562), Konrad L. Feng (10711565), Jason J. Herbert (10711568), Hafsa Rana (8201886), Kevin Danastas (10711571), Monica Miranda-Saksena (250011), Jake W. Rhodes (8506401), Ellis Patrick (4219258), Ralph C. Cohen (10711574), Jake Lim (10711577), Steven L. Merten (10711580), Andrew N. Harman (8506425), Anthony L. Cunningham (10668429) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Medicine, Microbiology, Cell Biology, Immunology, Cancer, Infectious Diseases, Virology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Epi-cDC 2s, uptake, HVEM, Isolated Epi-cDC 2s support, Virus type 1 infects Langerhans cells, epidermal MNP population, herpes simplex virus, guide HSV vaccine development, epidermal explants topically, entry pathways Skin, infection, LC, HIV, novel epidermal dendritic cell, HSV entry receptors nectin, dendritic cells type 2 |
| الوصف: |
A mixed population of epidermal MNPs was isolated from human abdominal epidermis and inoculated with HSV-1 GFP (MOI of 10) or mock treated for 1 h, then washed and incubated for a total infection time of 18 h (B & C) or 6 and 18 h (D & E) in HaCaT-conditioned medium at 37°C. The cells were then washed with PBS and labelled with fixable LIVE/DEAD Near-IR followed by antibodies to HLA-DR, CD45, CD1a, CD11c, and Langerin, then analysed by flow cytometry. HSV-1 infection was detected using GFP expression. (A) Representative gating strategy to distinguish LCs and Epi-cDC2s. CD45 + HLA-DR + cells were gated on and separated into CD1a + CD11c - LCs and CD1a low CD11c + Epi-cDC2s. Live cells from each subset were then assessed for HSV infection by GFP expression for which a representative donor is shown in (B). (C) The percentage of HSV-1 GFP + cells at 18 h in LCs (purple) and Epi-cDC2s (orange) is shown in box and whisker plots, where the central bar is the median, the box limits represent the upper and lower quartiles, the whiskers are the minimum and maximum values, and each dot represents an individual donor. A two-tailed paired t-test was used to compare the percentage of HSV-1 GFP + LCs and Epi-cDC2s, n = 17 . (D) Representative histograms of GFP expression at 6 and 18 h in LCs and Epi-cDC2s from one donor. (E) The expression of HSV-1 GFP, shown as gMFI, and the percentage of HSV-1 GFP + cells for LCs (purple dots) and Epi-cDC2s (orange dots) at 6 and 18 h, time points are paired by donor. Repeated measures two-way ANOVA with Bonferroni’s test for pairwise comparisons were used to compare the gMFI or the % HSV-1 GFP + LCs and Epi-cDC2s at 6 vs. 18 h, and LCs vs. Epi-cDC2s at each time point, n = 4. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Epi-cDC2s_are_more_extensively_infected_with_HSV-1_than_LCs_/14497394 |
| DOI: |
10.1371/journal.ppat.1009536.g002 |
| الاتاحة: |
https://doi.org/10.1371/journal.ppat.1009536.g002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.87E07F82 |
| قاعدة البيانات: |
BASE |