Academic Journal
Multiple and cooperative binding of fluorescence light-up probe thioflavin t with human telomere DNA G-quadruplex
| العنوان: | Multiple and cooperative binding of fluorescence light-up probe thioflavin t with human telomere DNA G-quadruplex |
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| المؤلفون: | Gabelica, Valerie, Maeda, R., Fujimoto, T., Yaku, H., Murashima, T., Sugimoto, N., Miyoshi, D. |
| المصدر: | Biochemistry, 52 (33), 5620-5628 (2013) |
| بيانات النشر: | American Chemical Society |
| سنة النشر: | 2013 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | Cooperative binding, Dissociation constant, Electrospray ionization mass spectrometry, Fluorescence titrations, Fluorescent probes, G-quadruplex structure, Human telomere DNA, Telomeric sequences, Biochemistry, Dissociation, Fluorescence, Ligands, Mass spectrometry, Probes, DNA sequences, DNA, DNA sequence, Algorithms, Binding Sites, Circular Dichroism, Fluorescent Dyes, G-Quadruplexes, Humans, Kinetics, Models, Molecular, Molecular Structure, Spectrometry, Mass, Electrospray Ionization |
| الوصف: | peer reviewed ; Thioflavin T (ThT), a typical probe for protein fibrils, also binds human telomeric G-quadruplexes with a fluorescent light-up signal change and high specificity against DNA duplexes. Cell penetration and low cytotoxicity of fibril probes having been widely established, modifying ThT and other fibril probes is an attractive means of generating new G-quadruplex ligands. Thus, elucidating the binding mechanism is important for the design of new drugs and fluorescent probes based on ThT. Here, we investigated the binding mechanism of ThT with several variants of the human telomeric sequence in the presence of monovalent cations. Fluorescence titrations and electrospray ionization mass spectrometry (ESI-MS) analyses demonstrated that each G-quadruplex unit cooperatively binds to several ThT molecules. ThT brightly fluoresces when a single ligand is bound to the G-quadruplex and is quenched as ligand binding stoichiometry increases. Both the light-up signal and the dissociation constants are exquisitely sensitive to the base sequence and to the G-quadruplex structure. These results are crucial for the sensible design and interpretation of G-quadruplex detection assays using fluorescent ligands in general and ThT in particular. © 2013 American Chemical Society. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0006-2960 1520-4995 |
| Relation: | urn:issn:0006-2960; urn:issn:1520-4995; https://orbi.uliege.be/handle/2268/202963; info:hdl:2268/202963; info:pmid:23909872 |
| DOI: | 10.1021/bi4006072 |
| الاتاحة: | https://orbi.uliege.be/handle/2268/202963 https://orbi.uliege.be/bitstream/2268/202963/1/bi-2013-006072-author%20postprint.pdf https://doi.org/10.1021/bi4006072 |
| Rights: | open access ; http://purl.org/coar/access_right/c_abf2 ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.87CE72B4 |
| قاعدة البيانات: | BASE |
| تدمد: | 00062960 15204995 |
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| DOI: | 10.1021/bi4006072 |