Academic Journal
Cathepsin K maintains the compartment of bone marrow T lymphocytes in vivo
العنوان: | Cathepsin K maintains the compartment of bone marrow T lymphocytes in vivo |
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المؤلفون: | Hausinger, Renate, Hackl, Marianne, Jardon Alvarez, Ana, Kehr, Miriam, Romero Marquez, Sandra, Hettler, Franziska, Kehr, Christian, Grziwok, Sandra, Schreck, Christina, Peschel, Christian, Istvánffy, Rouzanna, Oostendorp, Robert A. J. |
المساهمون: | Deutsche Forschungsgemeinschaft |
المصدر: | Immunity, Inflammation and Disease ; volume 9, issue 2, page 521-532 ; ISSN 2050-4527 2050-4527 |
بيانات النشر: | Wiley |
سنة النشر: | 2021 |
المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
الوصف: | In this study, we investigated the influence of the loss of cathepsin K ( Ctsk) gene on the hematopoietic system in vitro and in vivo. We found that cultures with lineage − SCA1 + KIT + (LSK) cells on Ctsk deficient stromal cells display reduced colony formation and proliferation, with increased differentiation, giving rise to repopulating cells with reduced ability to repopulate the donor LSKs and T cell compartments in the bone marrow (BM). Subsequent in vivo experiments showed impairment of lymphocyte numbers, but, gross effects on early hematopoiesis or myelopoiesis were not found. Most consistently in in vivo experimental settings, we found a significant reduction of (donor) T cell numbers in the BM. Lymphocyte deregulation is also found in transplantation experiments, which revealed that Ctsk is required for optimal regeneration of small populations of T cells, particularly in the BM, but also of thymic B cells. Interestingly, cell nonautonomous Ctsk regulates both B and T cell numbers, but T cell numbers in the BM require an additional autonomous Ctsk ‐dependent process. Thus, we show that Ctsk is required for the maintenance of hematopoietic stem cells in vitro, but in vivo, Ctsk deficiency most strongly affects lymphocyte homeostasis, particularly of T cells in the BM. |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | English |
DOI: | 10.1002/iid3.412 |
الاتاحة: | http://dx.doi.org/10.1002/iid3.412 https://onlinelibrary.wiley.com/doi/pdf/10.1002/iid3.412 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/iid3.412 |
Rights: | http://creativecommons.org/licenses/by/4.0/ |
رقم الانضمام: | edsbas.87C0D2F4 |
قاعدة البيانات: | BASE |
DOI: | 10.1002/iid3.412 |
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