التفاصيل البيبلوغرافية
| العنوان: |
Co-Occurrence of Multidrug Resistant Klebsiella pneumoniae Pathogenic Clones of Human Relevance in an Equine Pneumonia Case |
| المؤلفون: |
Venturini, Carola, Bowring, Bethany, Partridge, Sally R., Ben Zakour, Nouri L., Fajardo-Lubian, Alicia, Ayala Lopez, Ariana, Qin, Jilong, Totsika, Makrina, van Galen, Gaby, Norris, Jacqueline, Iredell, Jonathan |
| المصدر: |
Microbiology Spectrum |
| بيانات النشر: |
American Society for Microbiology |
| سنة النشر: |
2022 |
| المجموعة: |
Queensland University of Technology: QUT ePrints |
| مصطلحات موضوعية: |
bacteriophages, Klebsiella pneumoniae, multidrug resistance, pathogenic clones, plasmids, ST307 |
| الوصف: |
The global epidemiology of multidrug resistant Klebsiella pneumoniae, a serious threat to both animal and human health, is dominated by the spread of pathogenic clones, each separately evolving via acquisition of transferable antibiotic resistance or niche-specific virulence determinants. In horses, K. pneumoniae infection can lead to severe respiratory illness. Here, we characterized multiple isolates recovered from bronchial aspirates of a mare with pneumonia refractory to antibiotics. First, we used a combination of standard microbiology, bacteriophage cross-susceptibility and antibiotic resistance testing to profile the infecting K. pneumoniae population. The genomes of isolates with distinct fingerprints (pulsed-field gel electrophoresis) and unique combined bacteriophage/antibiotic profiles were then further analyzed using whole-genome sequencing. Adhesion to human epithelial cells and biofilm production were also measured as virulence indicators. Although it is commonly expected for one clone to dominate an infection episode, we identified five coexisting multidrug resistant K. pneumoniae sharing the same niche. One was a novel sequence type (ST4656), while the other four were all members of emerging human pathogenic clonal groups (ST307, ST628, ST893 and ST392). These isolates did not display significant differences from one another in terms of virulence or resistance and differed only in plasmid content from isolates implicated in severe human infections, with equal potential to prolong duration and severity of infection when sharing the same niche. This study highlights the importance of more precise surveillance and detection measures to uncover bacterial heterogeneity, reminding us that the “single clone” concept is not an absolute in invasive bacterial infections. IMPORTANCE Multidrug resistant Klebsiella pneumoniae are agents of life-threatening infections in animals and humans, with several multidrug resistant clones causing outbreaks of disease worldwide. It is generally accepted that only one ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| Relation: |
https://eprints.qut.edu.au/236762/1/118429181.pdf; Venturini, Carola, Bowring, Bethany, Partridge, Sally R., Ben Zakour, Nouri L., Fajardo-Lubian, Alicia, Ayala Lopez, Ariana, Qin, Jilong, Totsika, Makrina, van Galen, Gaby, Norris, Jacqueline, & Iredell, Jonathan (2022) Co-Occurrence of Multidrug Resistant Klebsiella pneumoniae Pathogenic Clones of Human Relevance in an Equine Pneumonia Case. Microbiology Spectrum, 10(3), Article number: e02158-21.; http://purl.org/au-research/grants/nhmrc/1144046; https://eprints.qut.edu.au/236762/; Centre for Immunology and Infection Control; Faculty of Health; School of Biomedical Sciences |
| الاتاحة: |
https://eprints.qut.edu.au/236762/ |
| Rights: |
free_to_read ; http://creativecommons.org/licenses/by/4.0/ ; 2022 The Authors ; This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
| رقم الانضمام: |
edsbas.878A96E9 |
| قاعدة البيانات: |
BASE |