Academic Journal
Interleukin 1 receptor antagonist gene variable number of tandem repeats polymorphism and cutaneous melanoma
| العنوان: | Interleukin 1 receptor antagonist gene variable number of tandem repeats polymorphism and cutaneous melanoma |
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| المؤلفون: | Cauci, Sabina, BULIGAN, Cinzia, Rocchi, Francesco, Salvador, Ilaria, Xodo, Luigi, Stinco, Giuseppe |
| المساهمون: | Cauci, Sabina, Buligan, Cinzia, Rocchi, Francesco, Salvador, Ilaria, Xodo, Luigi, Stinco, Giuseppe |
| سنة النشر: | 2019 |
| المجموعة: | Università degli Studi di Udine: CINECA IRIS |
| مصطلحات موضوعية: | cutaneous melanoma, skin cancer, polymorphism, interleukin 1 family, interleukin 1 receptor, interleukin 1 receptor antagonist, immunity, innate response |
| الوصف: | Immunity and cytokines serve crucial roles in cutaneous melanoma. The present study investigated whether a variable number tandem repeat (VNTR) polymorphism of interleukin-1 receptor antagonist (IL-1RA) gene (IL-1RN) located in intron 2 (rs2234663) is associated with cutaneous melanoma. A total of 515 subjects were studied, 133 of which were cutaneous melanoma cases (72 stage I+II non-metastatic melanoma cases and 61 stage III+IV metastatic melanoma cases), and 382 subjects were matching healthy controls from the Friuli-Venezia-Giulia Region located in Northeast Italy, an area with a high melanoma incidence. The IL-1RN-VNTR polymorphism was determined by DNA fragment length analysis following PCR amplification. According to the number of 86-bp repeats, five different IL-1RN alleles were identified: Allele 1 (4-repeats), allele 2 (2-repeats, short allele), allele 3 (5-repeats), allele 4 (3-repeats) and allele 5 (6-repeats). Alleles with three or more 86-bp repeats, i.e. allele 1, 3, 4 and 5 were collectively denoted as long (L) repeats. The present study revealed that IL-1RN-VNTR 1/2 and 2/L genotypes were more frequent among patients with cutaneous melanoma (43.6 and 45.1%, respectively) compared with healthy controls [29.6 and 30.6%, respectively; odds ratio (OR), 1.84; CI, 1.22-2.77; P=0.003; and OR, 1.66; CI, 1.24-2.79; P=0.002, respectively]. Conversely, the IL-1RN-VNTR 1/1 genotype was less frequent among melanoma cases (45.9%) compared with healthy controls (57.9%; OR, 0.62; CI, 0.41-0.92; P=0.017). Comparison of metastatic vs. non-metastatic melanoma cases identified no significant differences. The present study first demonstrated that carriage of the 1/1 IL-1RN-VNTR genotype was protective, whereas 1/2 and 2/L was a risk factor for patients with cutaneous melanoma vs. healthy controls. The short allele 2 was associated with higher expression levels of IL-1RA, a potent competitive inhibitor of the proinflammatory cytokines IL-1α and IL-1β. VNTR-IL-1RN polymorphism may affect susceptibility to melanoma ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000505576300012; volume:18; issue:6; firstpage:5759; lastpage:5768; numberofpages:10; journal:ONCOLOGY LETTERS; http://hdl.handle.net/11390/1167606; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85075652346 |
| DOI: | 10.3892/ol.2019.10923 |
| الاتاحة: | http://hdl.handle.net/11390/1167606 https://doi.org/10.3892/ol.2019.10923 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.875D1613 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3892/ol.2019.10923 |
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