Academic Journal
Formate‐tetrahydrofolate ligase: supplying the cytosolic one‐carbon network in roots with one‐carbon units originating from glycolate
| العنوان: | Formate‐tetrahydrofolate ligase: supplying the cytosolic one‐carbon network in roots with one‐carbon units originating from glycolate |
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| المؤلفون: | Saeheng, Sompop, Bailes, Clayton, Bao, Han, Gashu, Kelem, Morency, Matt, Arlynn, Tana, Smertenko, Andrei, Walker, Berkley James, Roje, Sanja |
| المساهمون: | National Science Foundation |
| المصدر: | The Plant Journal ; volume 119, issue 5, page 2464-2483 ; ISSN 0960-7412 1365-313X |
| بيانات النشر: | Wiley |
| سنة النشر: | 2024 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | SUMMARY The metabolism of tetrahydrofolate (H 4 PteGlu n )‐bound one‐carbon (C 1 ) units (C 1 metabolism) is multifaceted and required for plant growth, but it is unclear what of many possible synthesis pathways provide C 1 units in specific organelles and tissues. One possible source of C 1 units is via formate‐tetrahydrofolate ligase, which catalyzes the reversible ATP‐driven production of 10‐formyltetrahydrofolate (10‐formyl‐H 4 PteGlu n ) from formate and tetrahydrofolate (H 4 PteGlu n ). Here, we report biochemical and functional characterization of the enzyme from Arabidopsis thaliana (AtFTHFL). We show that the recombinant AtFTHFL has lower K m and k cat values with pentaglutamyl tetrahydrofolate (H 4 PteGlu 5 ) as compared to monoglutamyl tetrahydrofolate (H 4 PteGlu 1 ), resulting in virtually identical catalytic efficiencies for the two substrates. Stable transformation of Arabidopsis plants with the EGFP‐tagged AtFTHFL, followed with fluorescence microscopy, demonstrated cytosolic signal. Two independent T‐DNA insertion lines with impaired AtFTHFL function had shorter roots compared to the wild type plants, demonstrating the importance of this enzyme for root growth. Overexpressing AtFTHFL led to the accumulation of H 4 PteGlu n + 5,10‐methylene‐H 4 PteGlu n and serine, accompanied with the depletion of formate and glycolate, in roots of the transgenic Arabidopsis plants. This metabolic adjustment supports the hypothesis that AtFTHFL feeds the cytosolic C 1 network in roots with C 1 units originating from glycolate, and that these units are then used mainly for biosynthesis of serine, and not as much for the biosynthesis of 5‐methyl‐H 4 PteGlu n , methionine, and S ‐adenosylmethionine. This finding has implications for any future attempts to engineer one‐carbon unit‐requiring products through manipulation of the one‐carbon metabolic network in non‐photosynthetic organs. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/tpj.16933 |
| الاتاحة: | http://dx.doi.org/10.1111/tpj.16933 https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.16933 |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.86E9C6B8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/tpj.16933 |
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