التفاصيل البيبلوغرافية
| العنوان: |
Interleukin-9 (IL-9) stimulates the proliferation of human myeloid leukemic cells |
| المؤلفون: |
RM Lemoli, A. Fortuna, A. Tafuri, M. Fogli, M. Amabile, MR Ricciardi, MT Petrucci, L. Bonsi, GP Bagnara, G. Visani, G. Martinelli, S. Tura, GRANDE, Alexis, FERRARI, Sergio |
| المساهمون: |
Rm, Lemoli, A., Fortuna, A., Tafuri, M., Fogli, M., Amabile, Grande, Alexi, Mr, Ricciardi, Mt, Petrucci, L., Bonsi, Gp, Bagnara, G., Visani, G., Martinelli, Ferrari, Sergio, S., Tura |
| سنة النشر: |
1996 |
| المجموعة: |
Archivio della ricerca dell'Università di Modena e Reggio Emilia (Unimore: IRIS) |
| مصطلحات موضوعية: |
interleukin-9, acute myeloid leukemia |
| الوصف: |
Human interleukin-9 (IL-9) stimulates the proliferation of primitive hematopoietic erythroid and pluripotent progenitor cells, as well as the growth of selected colony-stimulating factor (CSF)-dependent myeloid cell lines. To further address the role of IL-9 in the development of acute leukemia, we evaluated the proliferative response of three leukemic cell lines and 32 primary samples from acute myeloblastic leukemia (AML) patients to recombinant human (rh)-IL-9 alone and combined with rh-IL-3, granulocyte-macrophage CSF (GM-CSF), and stem cell factor ([SCF] c-kit ligand). The colony-forming ability of HL60, K562, and KG1 cells and fresh AML cell populations upon IL-9 stimulation was assessed by a clonogenic assay in methylcellulose, whereas the cell-cycle characteristics of leukemic samples were determined by the acridine-orange flow cytometric technique and the bromodeoxyuridine (BRDU) incorporation assay. In addition, the terminal deoxynucleotidyl transferase assay (TDTA) and standard analysis of DNA cleavage by gel electrophoresis were used to evaluate induction or prevention of apoptosis by IL-9. IL-9, as a single cytokine, at various concentrations stimulated the colony formation of the three myeloid cell lines under serum-containing and serum-free conditions, and this effect was completely abrogated by anti-IL-9 monoclonal antibodies (MoAbs). When tested on fresh AML samples, optimal concentrations of IL-9 resulted in an increase of blast colony formation in all the cases studied (mean +/- SEM: 19 +/- 10 colony-forming unit-leukemic [CFU-L]/10(5) cells plated in control cultures v 107 +/- 32 in IL-9-supplemented dishes, P < .02). IL-9 stimulated 36.8% of CFU-L induced by phytohemagglutinin-lymphocyte-conditioned medium (PHA-LCM), and it was the most effective CSF for promoting leukemic cell growth among those tested in this study (ie, SCF, IL-3, and GM-CSF). The proliferative activity of IL-9 was also observed when T-cell-depleted AML specimens were incubated with increasing concentrations of the ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
STAMPA |
| اللغة: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/8611712; info:eu-repo/semantics/altIdentifier/wos/WOS:A1996UH14200038; volume:87; firstpage:3852; lastpage:3859; journal:BLOOD; http://hdl.handle.net/11380/310676; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-15844372772 |
| الاتاحة: |
http://hdl.handle.net/11380/310676 |
| رقم الانضمام: |
edsbas.86E238A3 |
| قاعدة البيانات: |
BASE |