Academic Journal
Identification of specific regions in hepatitis C virus core, NS2 and NS5 A that genetically interact with p7 and co‐ordinate infectious virus production
| العنوان: | Identification of specific regions in hepatitis C virus core, NS2 and NS5 A that genetically interact with p7 and co‐ordinate infectious virus production |
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| المؤلفون: | Gouklani, H., Beyer, C., Drummer, H., Gowans, E. J., Netter, H. J., Haqshenas, G. |
| المساهمون: | University of Glasgow, The Rockefeller University, National Health and Medical Research Council, Australian Centre for HIV & Hepatitis, CASS Foundation |
| المصدر: | Journal of Viral Hepatitis ; volume 20, issue 4 ; ISSN 1352-0504 1365-2893 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2012 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Summary The p7 protein of hepatitis C virus ( HCV ) is a small, integral membrane protein that plays a critical role in virus replication. Recently, we reported two intergenotypic JFH 1 chimeric viruses encoding the partial or full‐length p7 protein of the HCV ‐A strain of genotype 1b ( GT 1b; Virology ; 2007; 360:134). In this study, we determined the consensus sequences of the entire polyprotein coding regions of the wild‐type JFH 1 and the revertant chimeric viruses and identified predominant amino acid substitutions in core ( K 74 M ), NS 2 ( T 23 N , H 99 P ) and NS 5A ( D 251 G ). Forward genetic analysis demonstrated that all single mutations restored the infectivity of the defective chimeric genomes suggesting that the infectious virus production involves the association of p7 with specific regions in core, NS 2 and NS 5 A . In addition, it was demonstrated that the NS 2 T23N facilitated the generation of infectious intergenotypic chimeric virus encoding p7 from GT 6 of HCV . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/jvh.12004 |
| الاتاحة: | http://dx.doi.org/10.1111/jvh.12004 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjvh.12004 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jvh.12004 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.86E12D4D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/jvh.12004 |
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