التفاصيل البيبلوغرافية
| العنوان: |
The atrnh1b/c mutant is embryo lethal. |
| المؤلفون: |
Lingling Cheng (11226510), Wenjie Wang (413433), Yao Yao (335996), Qianwen Sun (426693) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Developmental Biology, Cancer, Infectious Diseases, Plant Biology, Virology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, organellar genome maintenance, single-stranded DNA-binding protein., mitochondrial DNA, AtRNH 1B, mitochondrial genome, RNase H 1, Arabidopsis Plant mitochondrial genomes, embryogenesi, chloroplast AtRNH 1C, HR surveillance, OSB, ectopic HR, Mitochondrial RNase H 1 activity, IR, RNase H 1 AtRNH 1C, Ectopic HR activity, cells lack AtRNH 1B transport |
| الوصف: |
(A) Representative siliques of atrnh1b-1 and atrnh1c plants, and siliques from reciprocal crosses of Col-0 and atrnh1b 1c +/− . Scale bars, 200 μm. (B) The ratio of atrnh1b 1c +/− to atrnh1b-1 was examined in 50 plates, each containing approximately 150 plants. Seeds were harvested from atrnh1b 1c +/− plants, sown on 1/2 MS medium, and selected based on Basta resistance. Because the atrnh1c mutant is resistant to Basta, the live plants are atrnh1b 1c +/− , and the dead plants are atrnh1b-1 . (C) F2 plants of atrnh1b 1c +/− complemented with AtRNH1Bpro : AtRNH1B/AtRNH1BΔMTS-GFP that were sown on 1/2 MS medium and selected based on Basta and hygromycin resistance. The complemented plants are resistant to hygromycin. The green plants are heterozygous atrnh1b 1c +/− , and the yellow plants are homozygous atrnh1b / c . (D) Different phenotypes of embryos in abnormal seeds that failed to transition to the heart stage. Scale bars, 20 μm. (E) Transmission electron microscopy of globular embryos from Col-0 and atrnh1c plants. White arrowheads indicate internal cristae membranes. Scale bars, 500 nm. (F) Confocal microscopy of mitochondria (labeled with MitoTracker) in atrnh1b/c aborted seeds and Col-0 normal seeds. Magenta = MitoTracker. Scale bars, 10 μm. The data underlying this figure can be found in S1 Data. 1/2 MS, half strength Murashige & Skoog (MS); GFP, green fluorescent protein; MTS, mitochondrial targeting signal. (PPTX) |
| نوع الوثيقة: |
conference object |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/presentation/The_i_atrnh1b_c_i_mutant_is_embryo_lethal_/15101151 |
| DOI: |
10.1371/journal.pbio.3001357.s003 |
| الاتاحة: |
https://doi.org/10.1371/journal.pbio.3001357.s003 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.867AB7C5 |
| قاعدة البيانات: |
BASE |