التفاصيل البيبلوغرافية
| العنوان: |
Co-treatment with miR-21-5p inhibitor and Aurora kinase inhibitor reversine suppresses breast cancer progression by targeting sprouty RTK signaling antagonist 2 |
| المؤلفون: |
Yue Zhang (30585), Yaoyi Wang (4027283), Jun Xue (158739), Wanping Liang (11887507), Zhisheng Zhang (155705), Xiuming Yang (8347377), Zhifei Qiao (11887510), Yang Jiang (46459), Junping Wang (397708), Xuchen Cao (85784), Peng Chen (6514) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Medicine, Cell Biology, Molecular Biology, Physiology, Pharmacology, Developmental Biology, Cancer, Hematology, Virology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Human breast cancer, reversine, miR-21-5p, SPRY2 |
| الوصف: |
Numerous studies have reported the regulatory effects of miR-21-5p and reversine in human breast cancer (HBC). However, the mechanism of reversine and miR-21-5p has not been fully investigated in HBC. The aim of the current study was to assess the mechanism of action of reversine, with or without miR-21-5p, in HBC progression. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot results confirmed the upregulation of miR-21-5p and downregulation of sprouty RTK signaling antagonist 2 (SPRY2) in HBC. Bioinformatics analysis and luciferase assay identified the correlation between miR-21-5p and SPRY2. Cell function experiment results indicated a decrease in migration, proliferation, and invasion of HBC cells treated with miR-21-5p inhibitor and reversine; however, an increase in apoptosis was observed in these cells. Apoptotic ability was more enhanced and migration, proliferation, and invasion were more impaired in HBC cells treated with both miR-21-5p inhibitor and reversine than in those treated individually with either inhibitors. SPRY2, downstream of miR-21-5p, participated in HBC progression with reversine. Overall, our study proved that combining the miR-21-5p inhibitor with reversine produced a synergistic effect by regulating SPRY2, thereby limiting HBC progression. This knowledge might offer insights into the clinical therapy of HBC. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Co-treatment_with_miR-21-5p_inhibitor_and_Aurora_kinase_inhibitor_reversine_suppresses_breast_cancer_progression_by_targeting_sprouty_RTK_signaling_antagonist_2/17705032 |
| DOI: |
10.6084/m9.figshare.17705032.v1 |
| الاتاحة: |
https://doi.org/10.6084/m9.figshare.17705032.v1 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.864F4EAD |
| قاعدة البيانات: |
BASE |