Academic Journal
Astragaloside-IV promotes autophagy via the Akt/mTOR pathway to improve cellular lipid deposition
| العنوان: | Astragaloside-IV promotes autophagy via the Akt/mTOR pathway to improve cellular lipid deposition |
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| المؤلفون: | Liu, Guo, Wang, Ye-Hui, Zhang, Ting, Li, Ya-Qiong, Chen, Xin-Yue, Dong, Wei, Li, Wei, Miao, Qi-Xiang, Qiao, Wen-Bo, Tian, Hui-Qiang, Yin, Shi-Long |
| المصدر: | Medicine ; volume 103, issue 16, page e37846 ; ISSN 0025-7974 1536-5964 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health) |
| سنة النشر: | 2024 |
| الوصف: | The current study aimed to investigate the potential role of astragaloside IV (AS-IV) in improving cellular lipid deposition and its underlying mechanism. A fatty liver cell model was established by treating hepatoma cells with palmitic acid. AS-IV and SC79 were used for treatment. Oil Red O staining was applied to detect intracellular lipid deposition, and transmission electron microscopy was utilized to assess autophagosome formation. Immunofluorescence double staining was applied to determine microtubule-associated proteins 1A/1B light chain 3 (LC3) expression. Western blot analysis was performed to detect the expression of LC3, prostacyclin, Beclin-1, V-akt murine thymoma viral oncogene homolog (Akt), phosphorylated Akt, mTOR, and phosphorylated mTOR. Oil Red O staining revealed that AS-IV reduced intracellular lipid accumulation. Further, it increased autophagosome synthesis and the expression of autophagy proteins LC3 and Beclin-1 in the cells. It also reduced the phosphorylation levels of Akt and mTOR and the levels of prostacyclin. However, the effects of AS-IV decreased with SC79 treatment. In addition, LC3B + BODIPY493/503 fluorescence double staining showed that AS-IV reduced intracellular lipid deposition levels by enhancing autophagy. AS-IV can reduce lipid aggregation in fatty liver cells, which can be related to enhanced hepatocyte autophagy by inhibiting the Akt/mTOR signaling pathway. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1097/md.0000000000037846 |
| DOI: | 10.1097/MD.0000000000037846 |
| الاتاحة: | http://dx.doi.org/10.1097/md.0000000000037846 https://journals.lww.com/10.1097/MD.0000000000037846 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.864B20A4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1097/md.0000000000037846 |
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