Academic Journal
Levels of Different Microbial Groups on Inert Surfaces of Poultry Slaughterhouses: Identification Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight and Detection of Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteria
| العنوان: | Levels of Different Microbial Groups on Inert Surfaces of Poultry Slaughterhouses: Identification Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight and Detection of Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteria |
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| المؤلفون: | Sarah Panera-Martínez, Cristina Rodríguez-Melcón, Daniel Rodríguez-Campos, Nuria Pérez-Estébanez, Rosa Capita, Carlos Alonso-Calleja |
| المصدر: | Antibiotics, Vol 13, Iss 7, p 587 (2024) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | poultry slaughterhouses, inert surfaces, microbial load, antibiotic resistance, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Knowledge of the microbiota present in food processing environments is a significant advance that will allow for better evaluation of the risk of food contamination and a better design of the procedures for sanitization. The levels of microbial group indicators of hygienic quality were determined in different areas of the slaughter lines of two poultry slaughterhouses in the northwest of Spain (22 surfaces in each slaughterhouse were studied). The average microbial levels (cfu/cm 2 ) were 2.15 × 10 2 ± 4.26 × 10 2 (total aerobic counts, TAC), 1.99 × 10 2 ± 5.00 × 10 2 (psychrotrophic microorganisms), 3.10 × 10 0 ± 1.37 × 10 1 (enterobacteria), 3.96 × 10 0 ± 2.55 × 10 1 (coliforms), 1.80 × 10 −1 ± 7.79 × 10 −1 (enterococci), and 1.12 × 10 −1 ± 3.35 × 10 −1 (vancomycin-resistant enterococci, VRE). TAC and psychrotrophic microorganisms were the most abundant groups in all samples ( p < 0.05). The counts of both microbial groups were higher ( p < 0.05) in samples of Slaughterhouse A than in those of Slaughterhouse B. Microbial loads for the rest of the bacteria were not influenced by the slaughterhouse sampled ( p > 0.05). All 44 samples showed TAC and psychrotrophic microorganisms. Colonies of the rest of the microbial groups were only found in 26 samples (59.1% of the total). The isolates (one from each sample) were identified with MALDI-TOF and PCR. Gram-negative bacteria (all Enterobacteriaceae ) were isolated in 23 samples, and Gram-positive bacteria were isolated in 16 (9 Enterococcus spp., 2 Enterococcus spp. and VRE, 3 VRE, 1 Enterococcus spp. and Listeria spp., and 1 Listeria spp.). The resistance of the strains to 11 ( Enterococcus spp.) or 17 ( Enterobacteriaceae ) antibiotics was determined (disk diffusion, CLSI), finding an average of 2.05 ± 2.06 resistances per strain (3.46 ± 2.27 if reduced susceptibility reactions are included). A total of 37.3% of the Enterobacteriaceae isolates had a gene for resistance to beta-lactam antibiotics ( bla TEM , bla CTX-M-15 , bla KPC , bla CMY -2 or bla NDM ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2079-6382 |
| Relation: | https://www.mdpi.com/2079-6382/13/7/587; https://doaj.org/toc/2079-6382; https://doaj.org/article/9b7027a815c5458aba39783d04253559 |
| DOI: | 10.3390/antibiotics13070587 |
| الاتاحة: | https://doi.org/10.3390/antibiotics13070587 https://doaj.org/article/9b7027a815c5458aba39783d04253559 |
| رقم الانضمام: | edsbas.86341C78 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20796382 |
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| DOI: | 10.3390/antibiotics13070587 |