التفاصيل البيبلوغرافية
| العنوان: |
Effect of Stevia rebaudiana Bertoni infusion on enamel demineralization and dental biofilm formation: an in-situ study ; Efeito da infusão de Stevia rebaudiana Bertoni na desmineralização do esmalte e na formação do biofilme dental: um estudo in situ |
| المؤلفون: |
Nied, Maieli Maiara, Arthur, Rodrigo Alex, de Freitas Farias, Ewelyn, Henz, Sandra Liana |
| المصدر: |
Revista da Faculdade de Odontologia de Porto Alegre; Vol. 63 No. 2 (2022); 12-23 ; Revista da Faculdade de Odontologia de Porto Alegre; v. 63 n. 2 (2022); 12-23 ; 2177-0018 ; 0566-1854 |
| بيانات النشر: |
UFRGS |
| سنة النشر: |
2023 |
| المجموعة: |
SEER UFRGS (Universidade Federal do Rio Grande do Sul: Sistema Eletrônico de Editoração de Periódic) |
| مصطلحات موضوعية: |
Stevia, Biofilms, Dental enamel, Hardness, Demineralization, Biofilmes, Esmalte dentário, Dureza, Desmineralização |
| الوصف: |
Aim: Stevia rebaudiana Bertoni (Stevia) is a natural non-caloric sweetener that can modify the cariogenicity of biofilms. This study aimed to evaluate the effect of Stevia infusion in microbial and biochemical composition of biofilms formed in the presence of sucrose and on enamel demineralization. Materials and Methods: In a cross-over design, eleven volunteers wore an intraoral palatal appliance containing 4 slabs of bovine enamel during 3 phases of 7 days each. Sucrose solution (20%) was dripped onto slabs 8 times/day and 0.9% sodium chloride (NaCl), 0.12% chlorhexidine (CHX), or 5% infusion of Stevia were dripped 2x/day. Biofilm formed on the slabs was collected and analyzed for counts of microorganisms (total bacteria, Lactobacilli, Candida spp., and Streptococcus mutans) biochemical composition in terms of soluble and insoluble extracellular polysaccharides and qualitative assessment by scanning electron microscopy. The percentage of surface hardness loss (%SHL) was determined on enamel slabs taken baseline and post-biofilm Knoop surface hardness values. Results: The % SHL in the CHX treatment was statistically lower in comparison to NaCl (p < 0.05). No differences were found between Stevia and CHX and between Stevia and NaCl. No other difference was found among the experimental groups with respect to the other outcomes. Discussion: Under high cariogenic conditions resembling frequent exposure to sucrose and absence of mechanical disruption, use of Stevia can neither modify the counts of cariogenic microorganisms nor the concentration of extracellular polysaccharides on in situ formed biofilms. This may have occurred due to the exposure of the biofilm to high sucrose concentration for all treatments and the condition of the microorganism growth in situ, which may hinder the diffusion of substances through the thick biofilm. Conclusion: Biofilm exposed to a high cariogenic challenge and without mechanical disruption is not affected by an infusion of Stevia rebaudiana Bertoni. ; Objetivo: A Stevia ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://seer.ufrgs.br/index.php/RevistadaFaculdadeOdontologia/article/view/126520/89194; https://seer.ufrgs.br/index.php/RevistadaFaculdadeOdontologia/article/view/126520 |
| الاتاحة: |
https://seer.ufrgs.br/index.php/RevistadaFaculdadeOdontologia/article/view/126520 |
| Rights: |
Copyright (c) 2022 Maieli Maiara Nied, Rodrigo Alex Arthur, Ewelyn de Freitas Farias, Sandra Liana Henz ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: |
edsbas.85737D80 |
| قاعدة البيانات: |
BASE |