التفاصيل البيبلوغرافية
| العنوان: |
Peptide-Based Electrochemiluminescence Biosensors Using Silver Nanoclusters as Signal Probes and Pd-Cu 2 O Hybrid Nanoconcaves as Coreactant Promoters for Immunoassays |
| المؤلفون: |
Xianzhen Song (8441907), Tingting Wu (228837), Chuannan Luo (8351646), Lu Zhao (296389), Xiang Ren (358465), Yong Zhang (5893), Qin Wei (1482154) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Genetics, Molecular Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, hydrogen evolution reaction, fab fragment ), antigen binding site, short peptide ligand, highly efficient peptide, 2 –, 2 +, proposed biosensor provides, hybrid nanoconcaves served, ag ncs served, 2 , thus significantly improving, immunoassays metal nanoclusters, hybrid nanoconcaves, thus increasing, overall reaction, metal ncs, fc fragment, binding efficiency, trace analysis, thereby avoiding, targeted immobilization, specifically bind, specific enolase |
| الوصف: |
Metal nanoclusters (NCs) possess high light stability and biocompatibility because of their unique quantum size effect, which has gradually become a new type of electrochemiluminescence (ECL) nanomaterial for immunoassays. However, the luminescence efficiency of metal NCs is too low to meet the needs of trace analysis, which limits its application. Herein, Ag NCs served as signal probes and Pd-Cu 2 O hybrid nanoconcaves served as coreaction promoters, developing a highly efficient peptide-based biosensor for neuron-specific enolase (NSE) detection. Utilizing the reversible cycle of Cu + /Cu 2+ and the reduction characteristics of Pd NPs, Pd-Cu 2 O greatly accelerates the reduction of S 2 O 8 2– . Meanwhile, Pd-Cu 2 O has good hydrogen evolution activity, which promotes the generation of oxygen by improving the redox efficiency of the overall reaction, thus increasing the yield of active intermediates (OH • ) to promote the reduction of S 2 O 8 2– . Specially, this is an effective attempt to use the hydrogen evolution reaction (HER) to accelerate the ECL emission of the S 2 O 8 2– system. In addition, a short peptide ligand (NARKFYKGC, NFC) was developed to implement the targeted immobilization of antibodies, which can specifically bind to the Fc fragment of antibodies, thereby avoiding the occupation of the antigen binding site (Fab fragment). The introduction of NFC not only improves the binding efficiency of antibodies but also protects its bioactivity, thus significantly improving the sensitivity of the biosensor. Based on these strategies, the proposed biosensor provides a new perspective for the applications of metal NCs in ECL systems. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Peptide-Based_Electrochemiluminescence_Biosensors_Using_Silver_Nanoclusters_as_Signal_Probes_and_Pd-Cu_sub_2_sub_O_Hybrid_Nanoconcaves_as_Coreactant_Promoters_for_Immunoassays/16622825 |
| DOI: |
10.1021/acs.analchem.1c03002.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.analchem.1c03002.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.851F083F |
| قاعدة البيانات: |
BASE |