Academic Journal
Effects of YB1 on the cleavage activity of Cas9.
| العنوان: | Effects of YB1 on the cleavage activity of Cas9. |
|---|---|
| المؤلفون: | Ekaterina A. Maltseva, Inna A. Vasil’eva, Nina A. Moor, Daria V. Kim, Nadezhda S. Dyrkheeva, Mikhail M. Kutuzov, Ivan P. Vokhtantsev, Lilya M. Kulishova, Dmitry O. Zharkov, Olga I. Lavrik |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Biophysics, Biochemistry, Microbiology, Genetics, Molecular Biology, Immunology, Developmental Biology, Mental Health, Space Science, Astronomical and Space Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, dependent gene editing, streptococcus pyogenes <, dna damage signalling, dna break sensing, div >< p, enzyme mostly orthogonal, dsb sensor ), closest homolog poly, repair , mostly orthogonal, target dna, cellulo <, repair due, strand breaks, proteins involved, polymerase 2, polymerase 1, natural regulation |
| الوصف: | CRISPR/Cas9 system is а powerful gene editing tool based on the RNA-guided cleavage of target DNA. The Cas9 activity can be modulated by proteins involved in DNA damage signalling and repair due to their interaction with double- and single-strand breaks (DSB and SSB, respectively) generated by wild-type Cas9 or Cas9 nickases. Here we address the interplay between Streptococcus pyogenes Cas9 and key DNA repair factors, including poly(ADP-ribose) polymerase 1 (SSB/DSB sensor), its closest homolog poly(ADP-ribose) polymerase 2, Ku antigen (DSB sensor), DNA ligase I (SSB sensor), replication protein A (DNA duplex destabilizer), and Y-box binding protein 1 (RNA/DNA binding protein). None of those significantly affected Cas9 activity, while Cas9 efficiently shielded DSBs and SSBs from their sensors. Poly(ADP-ribosyl)ation of Cas9 detected for poly(ADP-ribose) polymerase 2 had no apparent effect on the activity. In cellulo , Cas9-dependent gene editing was independent of poly(ADP-ribose) polymerase 1. Thus, Cas9 can be regarded as an enzyme mostly orthogonal to the natural regulation of human systems of DNA break sensing and repair. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/Effects_of_YB1_on_the_cleavage_activity_of_Cas9_/24663228 |
| DOI: | 10.1371/journal.pone.0294683.s013 |
| الاتاحة: | https://doi.org/10.1371/journal.pone.0294683.s013 https://figshare.com/articles/journal_contribution/Effects_of_YB1_on_the_cleavage_activity_of_Cas9_/24663228 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.84F0279 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0294683.s013 |
|---|