Academic Journal
Second-line drug susceptibility breakpoints for Mycobacterium tuberculosis using the MODS assay
| العنوان: | Second-line drug susceptibility breakpoints for Mycobacterium tuberculosis using the MODS assay |
|---|---|
| المؤلفون: | Trollip, A. P., Moore, D., Coronel, J., Caviedes, L., Klages, S., Victor, T., Romancenco, E., Crudu, V., Ajbani, K., Vineet, V. P., Rodrigues, C., Jackson, R. L., Eisenach, K., Garfein, R. S., Rodwell, T. C., Desmond, E., Groessl, E. J., Ganiats, T. G., Catanzaro, A. |
| بيانات النشر: | International Union Against Tuberculosis and Lung Disease |
| سنة النشر: | 2014 |
| المجموعة: | Repositorio - UPCH (Universidad Peruana Cayetano Heredia) |
| مصطلحات موضوعية: | MODS, aminoglycosides, drug susceptibility testing, fluoroquinolones, tuberculosis, https://purl.org/pe-repo/ocde/ford#3.03.08 |
| الوصف: | OBJECTIVE: To establish breakpoint concentrations for the fluoroquinolones (moxifloxacin [MFX] and ofloxacin [OFX]) and injectable second-line drugs (amikacin [AMK], kanamycin [KM] and capreomycin [CPM]) using the microscopic observation drug susceptibility (MODS) assay. SETTING: A multinational study conducted between February 2011 and August 2012 in Peru, India, Moldova and South Africa. DESIGN: In the first phase, breakpoints for the fluoroquinolones and injectable second-line drugs (n = 58) were determined. In the second phase, MODS second-line drug susceptibility testing (DST) as an indirect test was compared to MGIT™ DST (n = 89). In the third (n = 30) and fourth (n = 156) phases, we determined the reproducibility and concordance of MODS second-line DST directly from sputum. RESULTS: Breakpoints for MFX (0.5 μg/ml), OFX (1 μg/ml), AMK (2 μg/ml), KM (5 μg/ml) and CPM (2.5 μg/ml) were determined. In all phases, MODS results were highly concordant with MGIT DST. The few discrepancies suggest that the MODS breakpoint concentrations for some drugs may be too low. CONCLUSION: MODS second-line DST yielded comparable results to MGIT second-line DST, and is thus a promising alternative. Further studies are needed to confirm the accuracy of the drug breakpoints and the reliability of MODS second-line DST as a direct test. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1815-7920 |
| Relation: | urn:issn:1815-7920; https://hdl.handle.net/20.500.12866/10529; International Journal of Tuberculosis and Lung Disease; https://doi.org/10.5588/ijtld.13.0229 |
| DOI: | 10.5588/ijtld.13.0229 |
| الاتاحة: | https://hdl.handle.net/20.500.12866/10529 https://doi.org/10.5588/ijtld.13.0229 |
| Rights: | info:eu-repo/semantics/restrictedAccess ; https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
| رقم الانضمام: | edsbas.84D37731 |
| قاعدة البيانات: | BASE |
| تدمد: | 18157920 |
|---|---|
| DOI: | 10.5588/ijtld.13.0229 |