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Image_2_Neutrophil L-Plastin Controls Ocular Paucibacteriality and Susceptibility to Keratitis.JPEG
| العنوان: | Image_2_Neutrophil L-Plastin Controls Ocular Paucibacteriality and Susceptibility to Keratitis.JPEG |
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| المؤلفون: | Xiaoxiao Lu, Abirami Kugadas, Kirsten Smith-Page, Jeffrey Lamb, Tiffany Lin, Yusha Ru, Sharon Celeste Morley, Raina Fichorova, Sharad K. Mittal, Sunil K. Chauhan, Sejiro Littleton, Daniel Saban, Mihaela Gadjeva |
| سنة النشر: | 2020 |
| المجموعة: | Frontiers: Figshare |
| مصطلحات موضوعية: | Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, commensals, neutrophils, keratritis, P. aeruginosa, macrophages, infection |
| الوصف: | Why ocular mucosa is paucibacterial is unknown. Many different mechanisms have been suggested but the comprehensive experimental studies are sparse. We found that a deficiency in L-plastin (LCP1), an actin bundling protein, resulted in an ocular commensal overgrowth, characterized with increased presence of conjunctival Streptococcal spp. The commensal overgrowth correlated with susceptibility to P. aeruginosa-induced keratitis. L-plastin knock-out (KO) mice displayed elevated bacterial burden in the P. aeruginosa-infected corneas, altered inflammatory responses, and compromised bactericidal activity. Mice with ablation of LPL under the LysM Cre (LysM. Cre pos LPL fl/fl ) and S100A8 Cre (S100A8.Cre pos LPL fl/fl ) promoters had a similar phenotype to the LPL KOs mice. In contrast, infected CD11c.Cre pos LPL fl/fl mice did not display elevated susceptibility to infection, implicating the myeloid L-plastin-sufficient cells (e.g., macrophages and neutrophils) in maintaining ocular homeostasis. Mechanistically, the elevated commensal burden and the susceptibility to infection were linked to defects in neutrophil frequencies at steady state and during infection and compromised bactericidal activities upon priming. Macrophage exposure to commensal organisms primed neutrophil responses to P. aeruginosa, augmenting PMN bactericidal capacity in an L-plastin dependent manner. Cumulatively, our data highlight the importance of neutrophils in controlling ocular paucibacteriality, reveal molecular and cellular events involved in the process, and suggest a link between commensal exposure and resistance to infection. |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/Image_2_Neutrophil_L-Plastin_Controls_Ocular_Paucibacteriality_and_Susceptibility_to_Keratitis_JPEG/12074622 |
| DOI: | 10.3389/fimmu.2020.00547.s002 |
| الاتاحة: | https://doi.org/10.3389/fimmu.2020.00547.s002 https://figshare.com/articles/figure/Image_2_Neutrophil_L-Plastin_Controls_Ocular_Paucibacteriality_and_Susceptibility_to_Keratitis_JPEG/12074622 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.84D238E6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2020.00547.s002 |
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