التفاصيل البيبلوغرافية
| العنوان: |
Additional file 1 of Targeted theranostic photoactivation on atherosclerosis |
| المؤلفون: |
Joon Woo Song (4667578), Jae Won Ahn (8275248), Min Woo Lee (589379), Hyun Jung Kim (182757), Dong Oh Kang (10101906), Ryeong Hyun Kim (11601034), Un Gyo Kang (11601037), Yeon Hoon Kim (11601040), Jeongmoo Han (11601043), Ye Hee Park (11601046), Hyeong Soo Nam (4667584), Hongki Yoo (4664779), Kyeongsoon Park (1814110), Jin Won Kim (224828) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Medicine, Pharmacology, Immunology, Hematology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Macrophage targetable phototheranostic agent, Photodynamic therapy, Foam cells, Atherosclerosis, Autophagy, Efferocytosis |
| الوصف: |
Additional file 1: Figure S1. FT-IR spectra of (1) DS and (2) DS-Ce6. Figure S2. a Representative confocal microscopic images and quantification of dose-dependent cellular uptake of DS-Ce6 in the activated macrophages. *P < 0.05, ***P < 0.001. b Comparison of the intracellular uptake of free Ce6 (5 μM) and DS-Ce6 (equiv. 5 μM Ce6) in the activated macrophages. To evaluate receptor-mediated endocytosis, the SR-A ligand DS was pre-treated for 1 h before DS-Ce6 incubation (DS + DS-Ce6). ***P < 0.001. Scale bar = 50 μm. c Comparison of the intracellular uptake of DS-Ce6 (equiv. 5 μM Ce6) in ECs, SMCs, and RAW264.7 macrophages treated with LPS or LPS with LDL. ***P < 0.001 compared to ECs, and ###P < 0.001 compared to SMCs. Scale bar = 30 μm. d Viability of activated macrophages treated with different concentrations of DS-Ce6, DS, and Ce6 with laser irradiation (670 nm, 50 mW). **P < 0.01. e DS-Ce6 photoactivation-induced apoptosis according to the time after laser irradiation. Annexin V (green) and DAPI (blue)-stained images of the activated macrophages that received DS-Ce6 treatment (equiv. 5 μM Ce6) and laser irradiation (670 nm, 50 mW). ***P < 0.001. Scale bar = 50 μm. f DS-Ce6 photoactivation-induced autophagy flux according to the time after laser irradiation. IF staining of LC3 (green), p62 (red), and DAPI (blue) in the activated macrophages that received DS-Ce6 treatment (equiv. 5 μM Ce6) and laser irradiation (670 nm, 50 mW). Scale bar = 25 μm. ***P < 0.001. Figure S3. a Quantification of whole-body fluorescence in the lateral and prone positions at 0 and 5 min and 1, 3, 6, 9, 12, 24, and 48 h post-injection (n = 3). b Quantification of Ce6 fluorescence intensity within plaques treated with DS-Ce6 or free Ce6. *P < 0.05. c Co-localization of DS-Ce6 (CLSM) and macrophages (Mac3) within the atheroma (red arrow). Scale bar = 100 μm. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Additional_file_1_of_Targeted_theranostic_photoactivation_on_atherosclerosis/16864999 |
| DOI: |
10.6084/m9.figshare.16864999.v1 |
| الاتاحة: |
https://doi.org/10.6084/m9.figshare.16864999.v1 |
| Rights: |
CC BY + CC0 |
| رقم الانضمام: |
edsbas.847E6F84 |
| قاعدة البيانات: |
BASE |