Academic Journal
Engineered Protein Copolymers for Heparin Neutralization and Detection
| العنوان: | Engineered Protein Copolymers for Heparin Neutralization and Detection |
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| المؤلفون: | Liu, Qing, Shaukat, Ahmed, Meng, Zhoujun, Nummelin, Sami, Tammelin, Tekla, Kontturi, Eero, De Vries, Renko, Kostiainen, Mauri |
| المساهمون: | Department of Bioproducts and Biosystems, Biohybrid Materials, Materials Chemistry of Cellulose, VTT Technical Research Centre of Finland, Wageningen University and Research Centre, Aalto-yliopisto, Aalto University |
| بيانات النشر: | AMERICAN CHEMICAL SOCIETY |
| سنة النشر: | 2023 |
| المجموعة: | Aalto University Publication Archive (Aaltodoc) / Aalto-yliopiston julkaisuarkistoa |
| الوصف: | Heparin is a widely applied anticoagulant agent. However, in clinical practice, it is of vital importance to reverse its anticoagulant effect to restore the blood-clotting cascade and circumvent side effects. Inspired by protein cages that can encapsulate and protect their cargo from surroundings, we utilize three designed protein copolymers to sequester heparin into inert nanoparticles. In our design, a silk-like sequence provides cooperativity between proteins, generating a multivalency effect that enhances the heparin-binding ability. Protein copolymers complex heparin into well-defined nanoparticles with diameters below 200 nm. We also develop a competitive fluorescent switch-on assay for heparin detection, with a detection limit of 0.01 IU mL–1 in plasma that is significantly below the therapeutic range (0.2–8 IU mL–1). Moreover, moderate cytocompatibility is demonstrated by in vitro cell studies. Therefore, such engineered protein copolymers present a promising alternative for neutralizing and sensing heparin, but further optimization is required for in vivo applications. ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 1014-1021; application/pdf |
| اللغة: | English |
| تدمد: | 1525-7797 1526-4602 |
| Relation: | Biomacromolecules; Volume 24, issue 2; Liu, Q, Shaukat, A, Meng, Z, Nummelin, S, Tammelin, T, Kontturi, E, De Vries, R & Kostiainen, M 2023, ' Engineered Protein Copolymers for Heparin Neutralization and Detection ', Biomacromolecules, vol. 24, no. 2, pp. 1014-1021 . https://doi.org/10.1021/acs.biomac.2c01464; PURE UUID: 276aac00-9a2d-43c5-a706-06edbc692e4a; PURE ITEMURL: https://research.aalto.fi/en/publications/276aac00-9a2d-43c5-a706-06edbc692e4a; PURE LINK: http://www.scopus.com/inward/record.url?scp=85146034276&partnerID=8YFLogxK; PURE FILEURL: https://research.aalto.fi/files/102385738/CHEM_Liu_et_al_Engineered_Protein_Copolymers_2023_Biomacromolecules.pdf; https://aaltodoc.aalto.fi/handle/123456789/119989; URN:NBN:fi:aalto-202303072317 |
| DOI: | 10.1021/acs.biomac.2c01464 |
| الاتاحة: | https://aaltodoc.aalto.fi/handle/123456789/119989 https://doi.org/10.1021/acs.biomac.2c01464 |
| Rights: | openAccess |
| رقم الانضمام: | edsbas.84133F97 |
| قاعدة البيانات: | BASE |
| تدمد: | 15257797 15264602 |
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| DOI: | 10.1021/acs.biomac.2c01464 |