Academic Journal
Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp, nonstructural proteins p16 and p23, playing a role in virus replication
| العنوان: | Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp, nonstructural proteins p16 and p23, playing a role in virus replication |
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| المؤلفون: | Zhu, Jie, Miao, Qiuhong, Guo, Hongyuan, Tang, Aoxing, Dong, Dandan, Tang, Jingyu, Wang, Fang, Tong, Guangzhi, Liu, Guangqing |
| المصدر: | Virologica Sinica 37 (2022) 1 ; ISSN: 1674-0769 |
| سنة النشر: | 2022 |
| المجموعة: | Wageningen UR (University & Research Centre): Digital Library |
| مصطلحات موضوعية: | Nucleolin, RNA-dependent RNA polymerase (RdRp), Rabbit hemorrhagic disease virus (RHDV), Virus replication |
| الوصف: | Rabbit hemorrhagic disease virus (RHDV) is a member of the Caliciviridae family and cannot be propagated in vitro, which has impeded the progress of investigating its replication mechanism. Construction of an RHDV replicon system has recently provided a platform for exploring RHDV replication in host cells. Here, aided by this replicon system and using two-step affinity purification, we purified the RHDV replicase and identified its associated host factors. We identified rabbit nucleolin (NCL) as a physical link, which mediating the interaction between other RNA-dependent RNA polymerase (RdRp)-related host proteins and the viral replicase RdRp. We found that the overexpression or knockdown of NCL significantly increased or severely impaired RHDV replication in RK-13 cells, respectively. NCL was identified to directly interact with RHDV RdRp, p16, and p23. Furthermore, NCL knockdown severely impaired the binding of RdRp to RdRp-related host factors. Collectively, these results indicate that the host protein NCL is essential for RHDV replication and acts as a physical link between viral replicase and host proteins. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://edepot.wur.nl/566023 |
| DOI: | 10.1016/j.virs.2022.01.004 |
| الاتاحة: | https://research.wur.nl/en/publications/nucleolin-interacts-with-the-rabbit-hemorrhagic-disease-virus-rep https://doi.org/10.1016/j.virs.2022.01.004 |
| Rights: | https://creativecommons.org/licenses/by-nc-nd/4.0/ ; Wageningen University & Research |
| رقم الانضمام: | edsbas.83B85F4F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.virs.2022.01.004 |
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