التفاصيل البيبلوغرافية
| العنوان: |
Data_Sheet_1_Circulating Autoantibodies Recognizing Immunodominant Epitopes From Human Apolipoprotein B Associate With Cardiometabolic Risk Factors, but Not With Atherosclerotic Disease.docx |
| المؤلفون: |
Timoteo Marchini, Sara Malchow, Lourdes Caceres, Abed Al Hadi El Rabih, Sophie Hansen, Timothy Mwinyella, Lisa Spiga, Sven Piepenburg, Hauke Horstmann, Tijani Olawale, Xiaowei Li, Lucia Sol Mitre, Mark Colin Gissler, Heiko Bugger, Andreas Zirlik, Timo Heidt, Ingo Hilgendorf, Peter Stachon, Constantin von zur Muehlen, Christoph Bode, Dennis Wolf |
| سنة النشر: |
2022 |
| المجموعة: |
Frontiers: Figshare |
| مصطلحات موضوعية: |
Cardiology, Cardiology (incl. Cardiovascular Diseases), Cardiorespiratory Medicine and Haematology not elsewhere classified, atherosclerosis, cardiovascular disease, ApoB, auto-antibodies, immunity |
| الوصف: |
Rationale Atherosclerosis is a chronic inflammatory disease of large arteries that involves an autoimmune response with autoreactive T cells and auto-antibodies recognizing Apolipoprotein B (ApoB), the core protein of low-density lipoprotein (LDL). Here, we aimed to establish a clinical association between circulating human ApoB auto-antibodies with atherosclerosis and its clinical risk factors using a novel assay to detect auto-antibodies against a pool of highly immunogenic ApoB-peptides. Methods and Results To detect polyclonal IgM- and IgG-antibodies recognizing ApoB, we developed a chemiluminescent sandwich ELISA with 30 ApoB peptides selected by an in silico assay for a high binding affinity to MHC-II, which cover more than 80% of known MHC-II variants in a Caucasian population. This pre-selection of immunogenic self-peptides accounted for the high variability of human MHC-II, which is fundamental to allow T cell dependent generation of IgG antibodies. We quantified levels of ApoB-autoantibodies in a clinical cohort of 307 patients that underwent coronary angiography. Plasma anti-ApoB IgG and IgM concentrations showed no differences across healthy individuals (n = 67), patients with coronary artery disease (n = 179), and patients with an acute coronary syndrome (n = 61). However, plasma levels of anti-ApoB IgG, which are considered pro-inflammatory, were significantly increased in patients with obesity (p = 0.044) and arterial hypertension (p < 0.0001). In addition, patients diagnosed with the metabolic syndrome showed significantly elevated Anti-ApoB IgG (p = 0.002). Even when normalized for total plasma IgG, anti-ApoB IgG remained highly upregulated in hypertensive patients (p < 0.0001). We observed no association with triglycerides, total cholesterol, VLDL, or LDL plasma levels. However, total and normalized anti-ApoB IgG levels negatively correlated with HDL. In contrast, total and normalized anti-ApoB IgM, that have been suggested as anti-inflammatory, were significantly lower in diabetic ... |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/Data_Sheet_1_Circulating_Autoantibodies_Recognizing_Immunodominant_Epitopes_From_Human_Apolipoprotein_B_Associate_With_Cardiometabolic_Risk_Factors_but_Not_With_Atherosclerotic_Disease_docx/19569421 |
| DOI: |
10.3389/fcvm.2022.826729.s001 |
| الاتاحة: |
https://doi.org/10.3389/fcvm.2022.826729.s001
https://figshare.com/articles/dataset/Data_Sheet_1_Circulating_Autoantibodies_Recognizing_Immunodominant_Epitopes_From_Human_Apolipoprotein_B_Associate_With_Cardiometabolic_Risk_Factors_but_Not_With_Atherosclerotic_Disease_docx/19569421 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.839F8916 |
| قاعدة البيانات: |
BASE |