Academic Journal
Occurrence of Capnophilic Lactic Fermentation in the Hyperthermophilic Anaerobic Bacterium Thermotoga sp. Strain RQ7
| العنوان: | Occurrence of Capnophilic Lactic Fermentation in the Hyperthermophilic Anaerobic Bacterium Thermotoga sp. Strain RQ7 |
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| المؤلفون: | Esercizio, Nunzia, Lanzilli, Mariamichela, Landi, Simone, Caso, Lucio, Xu, Zhaohui, Nuzzo, Genoveffa, Gallo, Carmela, Manzo, Emiliano, Esposito, Sergio, Fontana, Angelo, d'Ippolito, Giuliana |
| المساهمون: | Esercizio, Nunzia, Lanzilli, Mariamichela, Landi, Simone, Caso, Lucio, Xu, Zhaohui, Nuzzo, Genoveffa, Gallo, Carmela, Manzo, Emiliano, Esposito, Sergio, Fontana, Angelo, D'Ippolito, Giuliana |
| سنة النشر: | 2022 |
| المجموعة: | IRIS Università degli Studi di Napoli Federico II |
| مصطلحات موضوعية: | ATPase, CO2 valorization, Thermotoga maritima, Thermotoga neapolitana, bio-based proce, green chemistry, hydrogen, lactic acid, Adenosine Triphosphatase, Adenosine Triphosphate, Anaerobiosi, Archaea, Base Composition, Fermentation, Phylogeny, Proton, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Carbon Dioxide, Thermotoga |
| الوصف: | Capnophilic lactic fermentation (CLF) is an anaplerotic pathway exclusively identified in the anaerobic hyperthermophilic bacterium Thermotoga neapolitana, a member of the order Thermotogales. The CO2-activated pathway enables non-competitive synthesis of hydrogen and L-lactic acid at high yields, making it an economically attractive process for bioenergy production. In this work, we discovered and characterized CLF in Thermotoga sp. strain RQ7, a naturally competent strain, opening a new avenue for molecular investigation of the pathway. Evaluation of the fermentation products and expression analyses of key CLF-genes by RT-PCR revealed similar CLF-phenotypes between T. neapolitana and T. sp. strain RQ7, which were absent in the non-CLF-performing strain T. maritima. Key CLF enzymes, such as PFOR, HYD, LDH, RNF, and NFN, are up-regulated in the two CLF strains. Another important finding is the up-regulation of V-ATPase, which couples ATP hydrolysis to proton transport across the membranes, in the two CLF-performing strains. The fact that V-ATPase is absent in T. maritima suggested that this enzyme plays a key role in maintaining the necessary proton gradient to support high demand of reducing equivalents for simultaneous hydrogen and lactic acid synthesis in CLF. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000867835900001; volume:23; issue:19; firstpage:12049; journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; https://hdl.handle.net/11588/902359; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85139931343 |
| DOI: | 10.3390/ijms231912049 |
| الاتاحة: | https://hdl.handle.net/11588/902359 https://doi.org/10.3390/ijms231912049 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.8363056B |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms231912049 |
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