Academic Journal
Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD
| العنوان: | Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD |
|---|---|
| المؤلفون: | Da-Ming Huang, Tian-Zhen Zhang, Feng-Jie Cui, Wen-Jing Sun, Li-Ming Zhao, Meng-Yi Yang, Ya-Juan Wang |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://downloads.hindawi.com/journals/bmri/2011/917232.pdf. |
| المجموعة: | CiteSeerX |
| الوصف: | A procedure for simultaneous identification and quantification of canrenone and its biotransformed product 11-α-hydroxycanrenone by high-performance liquid chromatography with ultraviolet detector (HPLC-UVD) and mass spectrometry (LC-MS) methods was proposed. The optimal determination variables on the HPLC-UVD or LC-MS coupled with a ZORBAX Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μm) were set as follows: detection wavelength of 280 nm, mobile phase of water and methanol gradient elution, temperature for the chromatographic column of 30 • C, flow rate of mobile phase of 0.8 mL/min, sample injection volume of 5 μL, and elution time of 40 min. The MS conditions were set as follows: the flow rate of sheath gas, aux gas, and sweep gas were kept at 35 arb, 5 arb, and 0 arb, respectively. The temperature of capillary was held at 300 • C, and capillary voltage was set at 30.00 V. Tube lens were performed at 100.00 V. The proposed method was validated by linearity (r 2 ≥ 0.9910), average recovery (94.93%, RSD1.21%), precision (RSD ≤ 1.31%), limit of detection, and limit of quantification (LOD 0.1∼0.12 mg/L, LOQ 0.5∼0.67 mg/L), which proved to be affordable for simultaneously determining canrenone and its bio-transformed product 11-α-hydroxy-canrenone. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1077.3700; http://downloads.hindawi.com/journals/bmri/2011/917232.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1077.3700 http://downloads.hindawi.com/journals/bmri/2011/917232.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.83323592 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |