Academic Journal
Capsid Lattice Destabilization Leads to Premature Loss of the Viral Genome and Integrase Enzyme during HIV-1 Infection
| العنوان: | Capsid Lattice Destabilization Leads to Premature Loss of the Viral Genome and Integrase Enzyme during HIV-1 Infection |
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| المؤلفون: | Eschbach, Jenna E., Elliott, Jennifer L., Li, Wen, Zadrozny, Kaneil K., Davis, Keanu, Mohammed, Shawn J., Lawson, Dana Q., Pornillos, Owen, Engelman, Alan N., Kutluay, Sebla B. |
| المساهمون: | Simon, Viviana, HHS | NIH | National Institute of Allergy and Infectious Diseases |
| المصدر: | Journal of Virology ; volume 95, issue 2 ; ISSN 0022-538X 1098-5514 |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2020 |
| الوصف: | The human immunodeficiency virus type 1 (HIV-1) capsid (CA) protein forms a conical lattice around the viral RNA genome and the associated viral enzymes and proteins, together constituting the viral core. Upon infection of a new cell, viral cores are released into the cytoplasm where they undergo a process termed “uncoating,” i.e., shedding of CA molecules from the conical lattice. Although proper and timely uncoating has been shown to be important for reverse transcription, the molecular mechanisms that link these two events remain poorly understood. In this study, we show that destabilization of the CA lattice leads to premature dissociation of CA from viral cores, which exposes the viral genome and the integrase enzyme for degradation in target cells. Thus, our studies demonstrate that the CA lattice protects the viral ribonucleoprotein complexes from untimely degradation in target cells and provide the first causal link between how CA stability affects reverse transcription. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/jvi.00984-20 |
| DOI: | 10.1128/JVI.00984-20 |
| الاتاحة: | http://dx.doi.org/10.1128/jvi.00984-20 https://journals.asm.org/doi/pdf/10.1128/JVI.00984-20 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.82EF2D33 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/jvi.00984-20 |
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