Academic Journal
Protein tyrosine kinase-dependent modulation of voltage-dependent potassium channels by genistein in rat cardiac ventricular myocytes
| العنوان: | Protein tyrosine kinase-dependent modulation of voltage-dependent potassium channels by genistein in rat cardiac ventricular myocytes |
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| المؤلفون: | Lau, CP, Li, GR, Wong, TM, Gao, Z |
| بيانات النشر: | //www.elsevier.com/locate/cellsig United States |
| سنة النشر: | 2004 |
| المجموعة: | University of Hong Kong: HKU Scholars Hub |
| مصطلحات موضوعية: | Potassium Channels, Voltage-Gated - metabolism, Animals, Enzyme Inhibitors - pharmacology, Genistein - pharmacology, Vanadates - pharmacology, Protein-Tyrosine Kinases - metabolism, Kinetics, Myocytes, Cardiac - metabolism, Rats, Heart Ventricles - cytology - metabolism |
| الوصف: | Effects of the isoflavone protein tyrosine kinase (PTK) inhibitor genistein on voltage-dependent K + currents, i.e., transient outward K + current (I to), sustained K + current (I ss), and inward rectifier K + current (I K1) were studied in rat cardiac ventricular myocytes. It was found that I to was reversibly inhibited by genistein in a concentration- dependent manner (IC 50=28.1 μM), while I ss was suppressed by genistein with IC 50 of 18.5 μM. In addition, I K1 (at -50 mV) was significantly decreased by 36.3±4.4% with 25 μM genistein. The inhibition of I to, I ss, and I K1 by genistein was significantly reversed by the application of the protein tyrosine phosphatase inhibitor sodium orthovanadate (1 mM). However, I to, I ss, and I K1 were not affected by the non-isoflavone PTK inhibitor tyrphostin A23 (100 μM) and PP2 (1 μM). These results indicate that activation of I to, I ss, and I K1 channels is modulated by genistein-sensitive PTKs in rat ventricular myocytes. © 2003 Elsevier Inc. All rights reserved. ; link_to_subscribed_fulltext |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0898-6568 |
| Relation: | Cellular Signalling; http://www.scopus.com/mlt/select.url?eid=2-s2.0-0347627268&selection=ref&src=s&origin=recordpage; Cellular Signalling, 2004, v. 16 n. 3, p. 333-341; 341; 88554; WOS:000187967900005; http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0898-6568&volume=16&issue=3&spage=333&epage=341&date=2004&atitle=Protein+tyrosine+kinase-dependent+modulation+of+voltage-dependent+potassium+channels+by+genistein+in+rat+cardiac+ventricular+myocytes; eid_2-s2.0-0347627268; 333; http://hdl.handle.net/10722/85477; 16 |
| DOI: | 10.1016/j.cellsig.2003.08.003 |
| الاتاحة: | https://doi.org/10.1016/j.cellsig.2003.08.003 http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0898-6568&volume=16&issue=3&spage=333&epage=341&date=2004&atitle=Protein+tyrosine+kinase-dependent+modulation+of+voltage-dependent+potassium+channels+by+genistein+in+rat+cardiac+ventricular+myocytes http://hdl.handle.net/10722/85477 |
| Rights: | Cellular Signalling. Copyright © Elsevier Inc. |
| رقم الانضمام: | edsbas.82CAF2C0 |
| قاعدة البيانات: | BASE |
| تدمد: | 08986568 |
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| DOI: | 10.1016/j.cellsig.2003.08.003 |