Academic Journal
REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish
| العنوان: | REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish |
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| المؤلفون: | Saiz, Nuria, Herrera Castillo, Lisbeth, Isorna Alonso, Esther, Delgado Saavedra, María Jesús, Conde Sieira, Marta, Soengas, José L., Pedro Ormeño, Nuria de |
| بيانات النشر: | MDPI |
| سنة النشر: | 2022 |
| المجموعة: | Universidad Complutense de Madrid (UCM): E-Prints Complutense |
| مصطلحات موضوعية: | 576:597.2/.5, fish, nr1d1, feeding, body weight, growth, metabolism, glucose, lipids, Biología celular (Biología), Peces, 2407 Biología Celular |
| الوصف: | REV-ERBα (nr1d1, nuclear receptor subfamily 1 group D member 1) is a transcriptional repressor that in mammals regulates nutrient metabolism, and has effects on energy homeostasis, although its role in teleosts is poorly understood. To determine REV-ERBα’s involvement in fish energy balance and metabolism, we studied the effects of acute and 7-day administration of its agonist SR9009 on food intake, weight and length gain, locomotor activity, feeding regulators, plasma and hepatic metabolites, and liver enzymatic activity. SR9009 inhibited feeding, lowering body weight and length gain. In addition, the abundance of ghrelin mRNA decreased in the intestine, and abundance of leptin-aI mRNA increased in the liver. Hypocretin, neuropeptide y (npy), and proopiomelanocortin (pomc) mRNA abundance was not modified after acute or subchronic SR9009 administration, while hypothalamic cocaine- and amphetamine-regulated transcript (cartpt-I) was induced in the subchronic treatment, being a possible mediator of the anorectic effects. Moreover, SR9009 decreased plasma glucose, coinciding with increased glycolysis and a decreased gluconeogenesis in the liver. Decreased triglyceride levels and activity of lipogenic enzymes suggest a lipogenesis reduction by SR9009. Energy expenditure by locomotor activity was not significantly affected by SR9009. Overall, this study shows for the first time in fish the effects of REV-ERBα activation via SR9009, promoting a negative energy balance by reducing energetic inputs and regulating lipid and glucose metabolism. ; REV-ERBα (nr1d1, receptor nuclear subfamilia 1 grupo D miembro 1) es un represor transcripcional que regula el metabolismo y la homeostasis energética en mamíferos. Sin embargo, su papel en teleósteos se conoce muy poco, por lo que el objetivo de este trabajo es conocer la implicación de REV-ERBα en el balance energético y metabólico en los peces. Para ello, se realizó una administración aguda y subcrónica de su agonista SR9009, estudiando los efectos sobre la ingesta, ganancia ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| Relation: | (PID2019-103969RBC32), (PID2019-103969RBC31); (PROEX 170.6/20); https://doi.org/10.3390/ijms23062921; https://hdl.handle.net/20.500.14352/72010 |
| DOI: | 10.3390/ijms23062921 |
| الاتاحة: | https://hdl.handle.net/20.500.14352/72010 https://doi.org/10.3390/ijms23062921 |
| Rights: | Atribución 3.0 España ; open access ; https://creativecommons.org/licenses/by/3.0/es/ |
| رقم الانضمام: | edsbas.82C4A579 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 |
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| DOI: | 10.3390/ijms23062921 |