Academic Journal
Lactate and short chain fatty acids produced by microbial fermentation downregulate proinflammatory responses in intestinal epithelial cells and myeloid cells.
| العنوان: | Lactate and short chain fatty acids produced by microbial fermentation downregulate proinflammatory responses in intestinal epithelial cells and myeloid cells. |
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| المؤلفون: | Iraporda, Carolina, Errea, Agustina, Romanin, David E, Cayet, Delphine, Pereyra, Elba, Pignataro, Omar, Sirard, Jean Claude, Garrote, Graciela L, Abraham, Analía G, Rumbo, Martín |
| المساهمون: | Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Consejo Nacional de Investigaciones Científicas y Técnicas Buenos Aires (CONICET)-Facultad de Ciencias Exactas La Plata, Universidad Nacional de la Plata Argentine (UNLP)-Universidad Nacional de la Plata Argentine (UNLP)-Comisión de Investigaciones Científicas Buenos Aires (CIC), Instituto de Estudios Inmunológicos y Fisiopatológicos Buenos Aires, Argentina (IIPF), Consejo Nacional de Investigaciones Científicas y Técnicas Buenos Aires (CONICET)-Universidad Nacional de la Plata Argentine (UNLP), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Instituto de Biología y Medicina Experimental Buenos Aires (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas Buenos Aires (CONICET), Facultad de Ciencias Exactas y Naturales Buenos Aires (FCEyN), Universidad de Buenos Aires Buenos Aires (UBA), CI and AE are fellows of Argentina National Research Council (CONICET), AGA, GLG, OP and MR are members of Scientific Career of CONICET, EP is member of CONICET. AE received a Bernardo Houssay fellowship from CONICET. Part of the work was supported by grants from Agencia Nacional de Promoción Científica y Tecnológica (ANPCYT) and ECOS A12B03 grant of the National Ministry of Science, Technology and Innovation (MINCYT-Argentina) and National Ministry of Science (France). |
| المصدر: | ISSN: 0171-2985 ; Immunobiology ; https://inserm.hal.science/inserm-01182910 ; Immunobiology, 2015, 220 (10), pp.1161-1169. ⟨10.1016/j.imbio.2015.06.004⟩. |
| بيانات النشر: | CCSD Elsevier |
| سنة النشر: | 2015 |
| المجموعة: | LillOA (HAL Lille Open Archive, Université de Lille) |
| مصطلحات موضوعية: | Immunomodulation, Inflammation, Innate immunity, Lactate, Short chain fatty acids, MESH: Animals, MESH: Bacteria/immunology, MESH: Intestinal Mucosa/microbiology, MESH: Lactic Acid/immunology, MESH: Mice, MESH: Myeloid Cells/immunology, MESH: Toll-Like Receptor 4/immunology, MESH: Toll-Like Receptor 5/immunology, MESH: CD40 Antigens/immunology, MESH: Caco-2 Cells, MESH: Down-Regulation/immunology, MESH: Epithelial Cells/immunology, MESH: Fatty Acids/immunology, MESH: Female, MESH: Humans, MESH: Intestinal Mucosa/immunology, [SDV.IMM]Life Sciences [q-bio]/Immunology |
| الوصف: | International audience ; The use of short chain fatty acids to modulate gastrointestinal inflammatory conditions such as ulcerative colitis has produced encouraging results either in animal models or also in clinical trials. Identifying the key cellular and molecular targets of this activity will contribute to establish the appropriate combinations/targeting strategies to maximize the efficacy of anti-inflammatory interventions. In the present work, we evaluated in vitro the interaction of lactate, acetate, propionate and butyrate on cells relevant for innate immune response of the gastrointestinal tract. All molecules tested regulate the production of proinflammatory cytokines by TLR-4 and TLR-5 activated intestinal epithelial cells in a dose response manner. Furthermore SCFAs and lactate modulate cytokine secretion of TLR-activated bone marrow derived macrophages and also TLR-dependent CD40 upregulation in bone marrow derived dendritic in a dose-dependent manner. Butyrate and propionate have been effective at concentrations of 1 to 5mM whereas acetate and lactate produced modulatory effects at concentrations higher than 20-50mM in different assays. Our results indicate that in concentrations similar to found in large bowel lumen, all SCFAs tested and lactate can modulate activity of relevant sentinel cell types activated by TLR signals. Modulatory activity was not inhibited by pertussis toxin treatment indicating that the effects are not related to Gi signaling. The use of these molecules in combined or separately as intervention strategy in conditions where epithelial or myeloid cells are main triggers of the inflammatory situation seems appropriate. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/26101138; PUBMED: 26101138 |
| DOI: | 10.1016/j.imbio.2015.06.004 |
| الاتاحة: | https://inserm.hal.science/inserm-01182910 https://doi.org/10.1016/j.imbio.2015.06.004 |
| رقم الانضمام: | edsbas.829A2B10 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.imbio.2015.06.004 |
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