التفاصيل البيبلوغرافية
| العنوان: |
Proximity-dependent biotin identification (BioID) of ATRX-associating proteins. |
| المؤلفون: |
William A. Scott (11707932), Erum Z. Dhanji (11707935), Boris J. A. Dyakov (11707938), Ema S. Dreseris (11707941), Jonathon S. Asa (11707944), Laura J. Grange (7848448), Mila Mirceta (11707947), Christopher E. Pearson (263866), Grant S. Stewart (11707950), Anne-Claude Gingras (154225), Eric I. Campos (11707953) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Medicine, Cell Biology, Genetics, Molecular Biology, Cancer, Hematology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, identify proximal associations, helicase protein associates, repetitive dna regions, dna repair factors, deposit histone h3, atrx proximal associations, daxx histone chaperone, new associating proteins, dependent chromatin remodelling, chromatin remodelling, histone deposition, dna replication, dependent biotinylation, associating proteins, chromatin maintenance, %22">xlink ">, used proximity, study expands, ribosome biosynthesis, proteomic changes |
| الوصف: |
A: Experimental pipeline. The BirA* biotin ligase was fused to ATRX to biotinylate proximal proteins. Labeled proteins were captured on streptavidin beads and analyzed by mass spectrometry. B: Dot plot showing prey proteins identified with ATRX-BirA* that were enriched over endogenous biotinylation (untransfected) and unspecific pan-cellular biotinylation (BirA* alone—BFDR ≤ 5%, SAINT [ 53 ]). Data represent two biological replicates. Proteins in boldface remained statistically enriched when the nuclear localization signal (NLS)-BirA* control was used to further filter the ATRX BioID data. C-D: Proximity-ligation assay (PLA) showing proximal associations between endogenous proteins in the MO3.13 human glial cell model. PLA data plots account for ∼100 nuclei in three independent experiments, with the exact number of nuclei assessed indicated in brackets. The p-values were obtained using a 2-sided Student’s t-test with unequal variance. Scale bars = 4 μm. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Proximity-dependent_biotin_identification_BioID_of_ATRX-associating_proteins_/17017293 |
| DOI: |
10.1371/journal.pgen.1009909.g001 |
| الاتاحة: |
https://doi.org/10.1371/journal.pgen.1009909.g001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.824F935C |
| قاعدة البيانات: |
BASE |