Academic Journal
Quantification of marine benthic communities with metabarcoding
| العنوان: | Quantification of marine benthic communities with metabarcoding |
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| المؤلفون: | Klunder, Lise, van Bleijswijk, Judith D. L., Kleine Schaars, Loran, van der Veer, Henk W., Luttikhuizen, Pieternella C., Bijleveld, Allert I. |
| المصدر: | Molecular Ecology Resources ; volume 22, issue 3, page 1043-1054 ; ISSN 1755-098X 1755-0998 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2021 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | DNA metabarcoding methods have been implemented in studies aimed at detecting and quantifying marine benthic biodiversity. In such surveys, universal barcodes are amplified and sequenced from environmental DNA. To quantify biodiversity with DNA metabarcoding, a relation between the number of DNA sequences of a species and its biomass and/or the abundance is required. However, this relationship is complicated by many factors, and it is often unknown. In this study, we validate estimates of biomass and abundance from molecular approaches with those from the traditional morphological approach. Abundance and biomass were quantified from 126 samples of benthic intertidal mudflat using traditional morphological approaches and compared with frequency of occurrence and relative read abundance estimates from a molecular approach. A relationship between biomass and relative read abundance was found for two widely dispersed annelid taxa ( Pygospio and Scoloplos ). None of the other taxons, however, showed such a relationship. We discuss how quantification of abundance and biomass using molecular approaches are hampered by the ecology of DNA i.e. all the processes that determine the amount of DNA in the environment, including the ecology of the benthic species as well as the compositional nature of sequencing data. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/1755-0998.13536 |
| الاتاحة: | http://dx.doi.org/10.1111/1755-0998.13536 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1755-0998.13536 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1755-0998.13536 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.81846CA5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/1755-0998.13536 |
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