Academic Journal
SNP discovery in complex allotetraploid genomes ( Gossypium spp., Malvaceae) using genotyping by sequencing
| العنوان: | SNP discovery in complex allotetraploid genomes ( Gossypium spp., Malvaceae) using genotyping by sequencing |
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| المؤلفون: | Logan‐Young, Carla Jo, Yu, John Z., Verma, Surender K., Percy, Richard G., Pepper, Alan E. |
| المصدر: | Applications in Plant Sciences ; volume 3, issue 3 ; ISSN 2168-0450 2168-0450 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2015 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Premise of the study: Single‐nucleotide polymorphism (SNP) marker discovery in plants with complex allotetraploid genomes is often confounded by the presence of homeologous loci (along with paralogous and orthologous loci). Here we present a strategy to filter for SNPs representing orthologous loci. Methods and Results: Using Illumina next‐generation sequencing, 54 million reads were collected from restriction enzyme–digested DNA libraries of a diversity of Gossypium taxa. Loci with one to three SNPs were discovered using the Stacks software package, yielding 25,529 new cotton SNP combinations, including those that are polymorphic at both interspecific and intraspecific levels. Frequencies of predicted dual‐homozygous (aa/bb) marker polymorphisms ranged from 6.7–11.6% of total shared fragments in intraspecific comparisons and from 15.0–16.4% in interspecific comparisons. Conclusions: This resource provides dual‐homozygous (aa/bb) marker polymorphisms. Both in silico and experimental validation efforts demonstrated that these markers are enriched for single orthologous loci that are homozygous for alternative alleles. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.3732/apps.1400077 |
| الاتاحة: | http://dx.doi.org/10.3732/apps.1400077 http://www.bioone.org/doi/full-xml/10.3732/apps.1400077 http://www.bioone.org/doi/pdf/10.3732/apps.1400077 |
| Rights: | http://creativecommons.org/licenses/by-nc/4.0/ |
| رقم الانضمام: | edsbas.817C27EF |
| قاعدة البيانات: | BASE |
| DOI: | 10.3732/apps.1400077 |
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