Academic Journal
Giant group I intron in a mitochondrial genome is removed by RNA back-splicing
| العنوان: | Giant group I intron in a mitochondrial genome is removed by RNA back-splicing |
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| المؤلفون: | Chi, Sylvia Ighem, Dahl, Mikael, Emblem, Åse, Johansen, Steinar |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2019 |
| المجموعة: | University of Tromsø: Munin Open Research Archive |
| مصطلحات موضوعية: | VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474, VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474, Amplexidiscus, Back-splicing, Catalytic RNA, Group I intron, Intron retention, Mitochondrial RNA, Ribozyme, Ricordea |
| الوصف: | Source at https://doi.org/10.1186/s12867-019-0134-y . ; Background - The mitochondrial genomes of mushroom corals (Corallimorpharia) are remarkable for harboring two complex group I introns; ND5-717 and COI-884. How these autocatalytic RNA elements interfere with mitochondrial RNA processing is currently not known. Here, we report experimental support for unconventional processing events of ND5-717 containing RNA. Results - We obtained the complete mitochondrial genome sequences and corresponding mitochondrial transcriptomes of the two distantly related corallimorpharian species Ricordea yuma and Amplexidiscus fenestrafer . All mitochondrial genes were found to be expressed at the RNA-level. Both introns were perfectly removed by autocatalytic splicing, but COI-884 excision appeared more efficient than ND5-717. ND5-717 was organized into giant group I intron elements of 18.1 kb and 19.3 kb in A. fenestrafer and R. yuma , respectively. The intron harbored almost the entire mitochondrial genome embedded within the P8 peripheral segment. Conclusion - ND5-717 was removed by group I intron splicing from a small primary transcript that contained a permutated intron–exon arrangement. The splicing pathway involved a circular exon-containing RNA intermediate, which is a hallmark of RNA back-splicing. ND5-717 represents the first reported natural group I intron that becomes excised by back-splicing from a permuted precursor RNA. Back-splicing may explain why Corallimorpharia mitochondrial genomes tolerate giant group I introns. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1471-2199 |
| Relation: | BMC Molecular Biology; FRIDAID 1712777; https://hdl.handle.net/10037/16238 |
| DOI: | 10.1186/s12867-019-0134-y |
| الاتاحة: | https://hdl.handle.net/10037/16238 https://doi.org/10.1186/s12867-019-0134-y |
| Rights: | openAccess |
| رقم الانضمام: | edsbas.81737227 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14712199 |
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| DOI: | 10.1186/s12867-019-0134-y |