التفاصيل البيبلوغرافية
| العنوان: |
Single-Cell RNA-Seq Reveals a Crosstalk between Hyaluronan Receptor LYVE-1-Expressing Macrophages and Vascular Smooth Muscle Cells |
| المؤلفون: |
Burger, Fabienne, Baptista, Daniela, Roth, Aline, Brandt, Karim, Fernandes Da Silva, Rafaela, Montecucco, Fabrizio, Mach, François, Miteva, Kapka Todorova |
| المصدر: |
ISSN: 2073-4409 ; Cells, vol. 11, no. 3 (2022) 411. |
| سنة النشر: |
2022 |
| المجموعة: |
Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: |
info:eu-repo/classification/ddc/616, CCL24, LYVE-1, VSMC transdifferentiation, osteogenic-like cells, resident-like macrophages, vascular calcification |
| الوصف: |
Background : Atherosclerosis is a chronic inflammatory disease where macrophages participate in the progression of the disease. However, the role of resident-like macrophages (res-like) in the atherosclerotic aorta is not completely understood. Methods: A single-cell RNA sequencing analysis of CD45 + leukocytes in the atherosclerotic aorta of apolipoprotein E-deficient ( Apoe -/- ) mice on a normal cholesterol diet (NCD) or a high cholesterol diet (HCD), respecting the side-to-specific predisposition to atherosclerosis, was performed. A population of res-like macrophages expressing hyaluronan receptor LYVE-1 was investigated via flow cytometry, co-culture experiments, and immunofluorescence in human atherosclerotic plaques from carotid artery disease patients (CAD). Results: We identified 12 principal leukocyte clusters with distinct atherosclerosis disease-relevant gene expression signatures. LYVE-1 + res-like macrophages, expressing a high level of CC motif chemokine ligand 24 (CCL24, eotaxin-2), expanded under hypercholesteremia in Apoe -/- mice and promoted VSMC phenotypic modulation to osteoblast/chondrocyte-like cells, ex vivo, in a CCL24-dependent manner. Moreover, the abundance of LYVE-1 + CCL24 + macrophages and elevated systemic levels of CCL24 were associated with vascular calcification and CAD events. Conclusions: LYVE-1 res-like macrophages, via the secretion of CCL24, promote the transdifferentiation of VSMC to osteogenic-like cells with a possible role in vascular calcification and likely a detrimental role in atherosclerotic plaque destabilization. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/35159221; https://archive-ouverte.unige.ch/unige:164697; unige:164697 |
| الاتاحة: |
https://archive-ouverte.unige.ch/unige:164697 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.81291536 |
| قاعدة البيانات: |
BASE |