Academic Journal
Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers
| العنوان: | Investigation of nano- and microdomains formed by ceramide 1 phosphate in lipid bilayers |
|---|---|
| المؤلفون: | Drabik, Dominik, Drab, Mitja, Penič, Samo, Iglič, Aleš, Czogalla, Aleksander |
| المصدر: | Scientific reports, vol. 13, number 18570, pp. 1-14, 2023. ; ISSN: 2045-2322 |
| بيانات النشر: | Nature Publishing Group |
| سنة النشر: | 2024 |
| المجموعة: | University of Ljubljana: Repository (RUJ) / Repozitorij Univerze v Ljubljani |
| مصطلحات موضوعية: | lipid bilayers, microdomains, Monte Carlo simulations, ceramide 1 phosphate, lipidni dvosloj, mikrodomene, Monte Carlo simulacije, ceramid 1 fosfat, info:eu-repo/classification/udc/577 |
| الوصف: | Biological membranes are renowned for their intricate complexity, with the formation of membrane domains being pivotal to the successful execution of numerous cellular processes. However, due to their nanoscale characteristics, these domains are often understudied, as the experimental techniques required for quantitative investigation present signifcant challenges. In this study we employ spotvariation z-scan fuorescence correlation spectroscopy (svzFCS) tailored for artifcial lipid vesicles of varying composition and combine this approach with high-resolution imaging. This method has been harnessed to examine the lipid-segregation behavior of distinct types of ceramide-1-phosphate (C1P), a crucial class of signaling molecules, within these membranes. Moreover, we provide a quantitative portrayal of the lipid membranes studied and the domains induced by C1P at both nano and microscales. Given the lack of defnitive conclusions from the experimental data obtained, it was supplemented with comprehensive in silico studies—including the analysis of difusion coefcient via molecular dynamics and domain populations via Monte Carlo simulations. This approach enhanced our insight into the dynamic behavior of these molecules within model lipid membranes, confrming that nano- and microdomains can co-exist in lipid vesicles. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf; text/url |
| اللغة: | English |
| Relation: | info:eu-repo/grantAgreement/ARIS//P2-0232-2022; info:eu-repo/grantAgreement/ARIS//J3-3066-2021; info:eu-repo/grantAgreement/other//J2-4447; https://repozitorij.uni-lj.si/IzpisGradiva.php?id=155446; https://repozitorij.uni-lj.si/Dokument.php?id=182335&dn=; https://repozitorij.uni-lj.si/Dokument.php?id=182333&dn=; https://plus.cobiss.net/cobiss/si/sl/bib/171022339; http://hdl.handle.net/20.500.12556/RUL-155446 |
| الاتاحة: | https://repozitorij.uni-lj.si/IzpisGradiva.php?id=155446 https://repozitorij.uni-lj.si/Dokument.php?id=182335&dn= https://repozitorij.uni-lj.si/Dokument.php?id=182333&dn= https://plus.cobiss.net/cobiss/si/sl/bib/171022339 https://hdl.handle.net/20.500.12556/RUL-155446 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.8113C355 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |