Academic Journal
Alkylated epidermal creatine kinase as a biomarker for sulfur mustard exposure: comparison to adducts of albumin and DNA in an in vivo rat study
| العنوان: | Alkylated epidermal creatine kinase as a biomarker for sulfur mustard exposure: comparison to adducts of albumin and DNA in an in vivo rat study |
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| المؤلفون: | Steinritz, Dirk, Lüling, Robin, Siegert, Markus, Herbert, Julia, Mückter, Harald, Taeger, Christian D., Gudermann, Thomas, Dietrich, Alexander, Thiermann, Horst, John, Harald |
| المصدر: | http://lobid.org/resources/99370671250806441#!, 95(4):1323-1333. |
| سنة النشر: | 2021 |
| المجموعة: | Publisso (ZB MED-Publikationsportal Lebenswissenschaften) |
| مصطلحات موضوعية: | Chemical Warfare Agents/toxicity [MeSH], Spectrometry, Mass, Electrospray Ionization [MeSH], Tandem Mass Spectrometry [MeSH], DNA Adducts/metabolism [MeSH], Humans [MeSH], Micro liquid chromatography, Cysteine/metabolism [MeSH], Skin/drug effects [MeSH], Rats [MeSH], High-resolution tandem-mass spectrometry, Animals [MeSH], Chromatography, Liquid [MeSH], Rats, Wistar [MeSH], Chemical warfare agents, Creatine Kinase/metabolism [MeSH], Alkylation/drug effects [MeSH], Male [MeSH], Mustard Gas/toxicity [MeSH], Biomarkers/metabolism [MeSH], Albumins/metabolism [MeSH], Human skin, Toxicokinetics and Metabolism, Skin/metabolism [MeSH] |
| الوصف: | Sulfur mustard (SM) is a chemical warfare agent which use is banned under international law and that has been used recently in Northern Iraq and Syria by the so-called Islamic State. SM induces the alkylation of endogenous proteins like albumin and hemoglobin thus forming covalent adducts that are targeted by bioanalytical methods for the verification of systemic poisoning. We herein report a novel biomarker, namely creatine kinase (CK) B-type, suitable as a local biomarker for SM exposure on the skin. Human and rat skin were proven to contain CK B-type by Western blot analysis. Following exposure to SM ex vivo, the CK-adduct was extracted from homogenates by immunomagnetic separation and proteolyzed afterwards. The cysteine residue Cys |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://repository.publisso.de/resource/frl:6451697; https://doi.org/10.1007/s00204-021-03005-3; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032612/ |
| DOI: | 10.1007/s00204-021-03005-3 |
| الاتاحة: | https://repository.publisso.de/resource/frl:6451697 https://doi.org/10.1007/s00204-021-03005-3 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032612/ |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.80B65FAC |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00204-021-03005-3 |
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