Academic Journal
Palmitoylation-dependent estrogen receptor alpha membrane localization: regulation by 17beta-estradiol
| العنوان: | Palmitoylation-dependent estrogen receptor alpha membrane localization: regulation by 17beta-estradiol |
|---|---|
| المؤلفون: | Paolo Ascenzi, Alessio Bocedi, Enzo Spisni, Vittorio Tomasi, Anna Trentalance, Paolo Visca, Maria Marino |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://www.molbiolcell.org/content/16/1/231.full.pdf. |
| سنة النشر: | 2005 |
| المجموعة: | CiteSeerX |
| الوصف: | A fraction of the nuclear estrogen receptor � (ER�) is localized to the plasma membrane region of 17�-estradiol (E2) target cells. We previously reported that ER � is a palmitoylated protein. To gain insight into the molecular mechanism of ER� residence at the plasma membrane, we tested both the role of palmitoylation and the impact of E2 stimulation on ER� membrane localization. The cancer cell lines expressing transfected or endogenous human ER � (HeLa and HepG2, respectively) or the ER � nonpalmitoylable Cys447Ala mutant transfected in HeLa cells were used as experimental models. We found that palmitoylation of ER � enacts ER � association with the plasma membrane, interaction with the membrane protein caveolin-1, and nongenomic activities, including activation of signaling pathways and cell proliferation (i.e., ERK and AKT activation, cyclin D 1 promoter activity, DNA synthesis). Moreover, E2 reduces both ER � palmitoylation and its interaction with caveolin-1, in a time- and dose-dependent manner. These data point to the physiological role of ER� palmitoylation in the receptor localization to the cell membrane and in the regulation of the E2-induced cell proliferation. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.335.7535; http://www.molbiolcell.org/content/16/1/231.full.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.335.7535 http://www.molbiolcell.org/content/16/1/231.full.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.80B53935 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |