Academic Journal
Antibodies Directed against a Peptide Epitope of a Klebsiella pneumoniae-Derived Protein Are Present in Ankylosing Spondylitis
| العنوان: | Antibodies Directed against a Peptide Epitope of a Klebsiella pneumoniae-Derived Protein Are Present in Ankylosing Spondylitis |
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| المؤلفون: | PUCCETTI, Antonio, Dolcino, Marzia, TINAZZI, Elisa, MORETTA, Francesca, D'Angelo, Salvatore, Olivieri, Ignazio, LUNARDI, Claudio |
| المساهمون: | Puccetti, Antonio, Dolcino, Marzia, Tinazzi, Elisa, Moretta, Francesca, D'Angelo, Salvatore, Olivieri, Ignazio, Lunardi, Claudio |
| سنة النشر: | 2017 |
| المجموعة: | Università degli Studi di Verona: Catalogo dei Prodotti della Ricerca (IRIS) |
| مصطلحات موضوعية: | Ankylosing spondylitis, Klebsiella pneumoniae |
| الوصف: | Ankylosing spondylitis (AS) is a chronic inflammatory arthritis of unknown origin. Its autoimmune origin has been suggested but never proven. Several reports have implicated Klebsiella pneumoniae as a triggering or perpetuating factor in AS; however, its role in the disease pathogenesis remains debated. Moreover, despite extensive investigations, a biomarker for AS has not yet been identified. To clarify these issues, we screened a random peptide library with pooled IgGs obtained from 40 patients with AS. A peptide (AS peptide) selected from the library was recognized by serum IgGs from 170 of 200 (85%) patients with AS but not by serum specimens from 100 healthy controls. Interestingly, the AS peptide shows a sequence similarity with several molecules expressed at the fibrocartilaginous sites that are primarily involved in the AS inflammatory process. Moreover, the peptide is highly homologous to a Klebsiella pneumoniae dipeptidase (DPP) protein. The antibody affinity purified against the AS peptide recognizes the autoantigens and the DPP protein. Furthermore, serum IgG antibodies against the Klebsiella DPP121-145 peptide epitope were detected in 190 of 200 patients with AS (95%), 3 of 200 patients with rheumatoid arthritis (1.5%) and only 1 of 100 (1%) patients with psoriatic arthritis. Such reactivity was not detected in healthy control donors. Our results show that antibodies directed against an epitope of a Klebsiella pneumoniae-derived protein are present in nearly all patients with AS. In the absence of serological biomarkers for AS, such antibodies may represent a useful tool in the diagnosis of the disease. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/28135336; info:eu-repo/semantics/altIdentifier/wos/WOS:000396124700045; volume:12; issue:1; firstpage:e0171073; lastpage:e0171073; numberofpages:1; journal:PLOS ONE; http://hdl.handle.net/11562/959913; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85011317377 |
| DOI: | 10.1371/journal.pone.0171073 |
| الاتاحة: | http://hdl.handle.net/11562/959913 https://doi.org/10.1371/journal.pone.0171073 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.8099159C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0171073 |
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