Academic Journal
Evidence for heterogeneity in response to treatment in mammary tumors of dogs as happens in humans
| العنوان: | Evidence for heterogeneity in response to treatment in mammary tumors of dogs as happens in humans |
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| المؤلفون: | Turna O., Uvez A., Baykal A., Sedef Develi E., Diramali M., Sonmez K., Karakas D. |
| بيانات النشر: | Springer Science and Business Media B.V. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | ATP assay, Canine, Chemosensitivity, Chemotherapy, Mammary tumor, cisplatin, doxorubicin, vincristine, adenosine triphosphate, antineoplastic agent, animal experiment, animal tissue, Article, breast tumor, cancer chemotherapy, cancer tissue, cell isolation, controlled study, dog, drug response, ex vivo study, female, follow up, genetic heterogeneity, human, hyperplasia, in vitro study, lymph node metastasis, nonhuman, statistically significant result |
| الوصف: | Tumors are formed by various clones developed over a long time. This gives rise to a heterogeneous nature. This heterogeneity is the hardest challenge in the treatment of cancers because it is the main reason for drug resistance. This is a well-known fact in human cancer. Therefore, we have reasoned that if the tumor heterogeneity in canine mammary gland tumors (CMGTs) could be shown by an ex vivo assay, which will be used first time in veterinary oncology practice, this could be used further in clinics. To achieve this, twenty-six patients were included in the study. Tumor tissues were obtained from animals during routine surgery. Tumor cells were isolated and seeded ex vivo. The cells were exposed to anticancer drugs that are clinically used. Seven days after the treatment, chemosensitivity has luminometrically been assayed by ATP-tumor chemosensitivity assay (ATP-TCA). It has clearly been shown that all the tumor tissues have responded to treatment differently, implying that heterogeneity exists in mammary tumors. There has also been found that there was a weak to moderate statistically significant correlation between tumor size and drug index. However, there has been no correlation between drug index and metastasis to lymph nodes. Hyperplasic areas had relatively higher PCNA values. The results of our study demonstrate the heterogeneity in responses to in vitro drugs. Clinical trials based on test results and follow-up studies with large numbers of animals are needed to prove that such chemotherapeutic activity assessment tests can be clinically useful in predicting drug responses in CMGTs. © 2022, The Author(s), under exclusive licence to Springer Nature B.V. ; Istanbul Üniversitesi-Cerrahpasa, IÜC: TSA-2018–28943 ; This work was supported by the Research Fund of Istanbul University-Cerrahpasa (Project number: TSA-2018–28943). Author Ozge Turna has received research support from Istanbul University-Cerrahpasa. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 01657380 |
| Relation: | Veterinary Research Communications; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://doi.org/10.1007/s11259-022-09934-3; https://hdl.handle.net/20.500.12831/19484; 47; 111; 120; 2-s2.0-85130110242 |
| DOI: | 10.1007/s11259-022-09934-3 |
| الاتاحة: | https://doi.org/10.1007/s11259-022-09934-3 https://hdl.handle.net/20.500.12831/19484 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.804E8551 |
| قاعدة البيانات: | BASE |
| تدمد: | 01657380 |
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| DOI: | 10.1007/s11259-022-09934-3 |