التفاصيل البيبلوغرافية
| العنوان: |
NMR, HPLC-ESI-MS, and MALDI-TOF MS analysis of condensed tannins from Delonix regia (Bojer ex Hook) Raf and their bioactivities |
| المؤلفون: |
Chai, Wei-Ming, Shi, Yan, Feng, Hui-Ling, Qiu, Ling, Zhou, Hai-Chao, Deng, Zi-Wei, Yan, Chong-Ling, Chen, Qing-Xi, 石艳, 严重玲, 陈清西 |
| المصدر: |
http://dx.doi.org/10.1021/jf300740d. |
| بيانات النشر: |
American Chemical Society |
| سنة النشر: |
2012 |
| المجموعة: |
Xiamen University Institutional Repository |
| مصطلحات موضوعية: |
Agents, Fruits, High performance liquid chromatography, Mass spectrometry, Nuclear magnetic resonance, Oligomers |
| الوصف: |
The structures of the condensed tannins isolated from leaf, fruit, and stem bark of Delonix regia (Bojer ex Hook.) Raf. have been investigated with 13C nuclear magnetic resonance (13C NMR) and high performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) coupled with thiolysis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analyses. The results showed that these condensed tannins from D. regia possessed structural heterogeneity in monomer units and degree of polymerization. Propelargonidin (PP) and procyanidin (PC) were found in the leaf, fruit, and stem bark of D. regia, while prodelphinidin (PD) was found only in the leaves. The polymer chain lengths of condensed tannins from leaf and fruit organs were detected to be trimers to hexadecamers but from trimers to tridecamers for stem bark. B-type linkages were present in all these compounds. Condensed tannins from different parts of D. regia can be explored as tyrosinase inhibitors and food antioxidants because of their potent antityrosinase and antioxidant activities. The inhibitor concentration leading to 50% enzyme activity (IC 50) was estimated to be 38 卤 1, 73 卤 2, and 54 卤 1.5 渭g/mL for the condensed tannins of leaf, fruit, and stem bark. Condensed tannins extracted from stem bark exhibited the highest antioxidant activity; the DPPH scavenging activity (IC50) and the FRAP values were 90 卤 2 渭g/mL and 5.42 卤 0.09 mmol AAE/g, respectively. 漏 2012 American Chemical Society. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
Journal of Agricultural and Food Chemistry, 2012,60(19):5013-5022; 20122115045642; http://dspace.xmu.edu.cn/handle/2288/90870 |
| الاتاحة: |
http://dspace.xmu.edu.cn/handle/2288/90870 |
| رقم الانضمام: |
edsbas.800A6054 |
| قاعدة البيانات: |
BASE |